Tested Applications
| Positive WB detected in | mouse kidney tissue, SMMC-7721 cells, HepG2 cells, mouse liver tissue, rat kidney tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26570-1-AP targets EHHADH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24237 Product name: Recombinant human EHHADH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 108-284 aa of BC038948 Sequence: GCHYRIAHAEAQVGLPEVTLGLLPGARGTQLLPRLTGVPAALDLITSGRRILADEALKLGILDKVVNSDPVEEAIRFAQRVSDQPLESRRLCNKPIQSLPNMDSIFSEALLKMRRQHPGCLAQEACVRAVQAAVQYPYEVGIKKEEELFLYLLQSGQARALQYAFFAERKANKWSTP Predict reactive species |
| Full Name | enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase |
| Calculated Molecular Weight | 723 aa, 79 kDa |
| Observed Molecular Weight | 69 kDa |
| GenBank Accession Number | BC038948 |
| Gene Symbol | EHHADH |
| Gene ID (NCBI) | 1962 |
| RRID | AB_2880556 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08426 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EHHADH antibody 26570-1-AP | Download protocol |
| IHC protocol for EHHADH antibody 26570-1-AP | Download protocol |
| WB protocol for EHHADH antibody 26570-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EHHADH Polyclonal antibody (Cat# 26570-1-AP) has 22 citations. Publications appear in journals including Cell Metabolism, Cell Research, Experimental & Molecular Medicine, Cell Discovery, Phytomedicine, Cell Reports, JCI Insight, Journal of Ethnopharmacology, Life Sciences, Geroscience, Communications Biology, International Journal of Molecular Sciences, Scientific Reports, Cancers, Histopathology, International Journal of Biochemistry & Cell Biology, Applied Biochemistry and Biotechnology, Molecular Pharmacology, American Journal of Physiology, and Genes & Genomics. Research fields span metabolic disorders (obesity, fatty liver, MASLD), hepatocellular carcinoma, breast cancer, mitochondrial/fatty acid metabolism, PPAR signaling, and oxidative stress.
| Species | Application | Title |
|---|---|---|
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
Cell Metab Maternal vitamin B12 deprivation exacerbates offspring obesity by reducing early-life colonization with Bifidobacterium pseudolongum. | ||
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation | ||
Exp Mol Med LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver | ||
Cell Discov A decrease in Flavonifractor plautii and its product, phytosphingosine, predisposes individuals with phlegm-dampness constitution to metabolic disorders | ||
Phytomedicine Qige Decoction attenuated non-alcoholic fatty liver disease through regulating SIRT6-PPARα-mediated fatty acid oxidation |
