EHMT2 Monoclonal antibody

• Home
• Products
• Primary Antibodies
• EHMT2 Monoclonal antibody (66689-1-Ig)

• Featured Product
• KD/KO Validated

EHMT2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66689-1-Ig

Clone No.1A12E3

Publications(12)

Reviews (2)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

EHMT2/G9a, 1A12E3, BAT8, C6orf30, EC:2.1.1.-

• 

  WB analysis of HeLa using 66689-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 66689-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 66689-1-Ig

  WB result of EHMT2 antibody (66689-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EHMT2 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 66689-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EHMT2 antibody (66689-1-Ig, Clone: 1A12E3 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66689-1-Ig targets EHMT2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27477

  Product name: Recombinant human EHMT2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 652-1001 aa of BC018718

  Sequence: ESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELGSLPPVNT

• Full Name: euchromatic histone-lysine N-methyltransferase 2
• Calculated Molecular Weight: 1210 aa, 132 kDa
• Observed Molecular Weight: 150 kDa and 170 kDa
• GenBank Accession Number: BC018718
• Gene Symbol: EHMT2
• Gene ID (NCBI): 10919
• RRID: AB_2882043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96KQ7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EHMT2 (Euchromatic Histone Lysine Methyltransferase 2), also known as G9a, is a key epigenetic regulatory enzyme. EHMT2 contains a SET domain, which is responsible for its methyltransferase activity. EHMT2 primarily functions by forming heterodimers with its homolog, EHMT1 (also known as GLP); together, these two proteins are responsible for the mono- and dimethylation of lysine 9 on histone H3 (H3K9me1 and H3K9me2).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human	WB	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan
human	WB	Am J Cancer Res; HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.; Authors - Li-Na Yang
human	WB	iScience; Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.; Authors - Jun Yamamoto
human	WB	bioRxiv; Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy; Authors - Zhihua Kang
		Comput Biol Chem; EHMT2 and INMT as methyltransferase related biomarkers predicting prognosis in HBV associated hepatocellular carcinoma.; Authors - Peilin Xie

Reviews

4.5 out of 5

2 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

aditya (Verified Customer) (03-24-2026)

great

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hek

Hongtao (Verified Customer) (12-17-2021)

This antibody works very well for human cancer cell whole cell lysate with 10 ug total protein input.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human kidney cancer cell

EHMT2 Antibody Western Blot validation (1:1000 dilution) in human kidney cancer cell (Cat no:66689-1-Ig)