EHMT2 Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 66689-1-Ig (same clone as 66689-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66689-1-Ig (same clone as 66689-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66689-1-Ig (same clone as 66689-1-PBS)

  WB result of EHMT2 antibody (66689-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EHMT2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66689-1-Ig (same clone as 66689-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EHMT2 antibody (66689-1-Ig, Clone: 1A12E3 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66689-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

EHMT2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 66689-1-PBS

Clone No.1A12E3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EHMT2/G9a, 1A12E3, BAT8, C6orf30, EC:2.1.1.-

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Product Information

66689-1-PBS targets EHMT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27477

  Product name: Recombinant human EHMT2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 652-1001 aa of BC018718

  Sequence: ESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELGSLPPVNT

• Full Name: euchromatic histone-lysine N-methyltransferase 2
• Calculated Molecular Weight: 1210 aa, 132 kDa
• Observed Molecular Weight: 150 kDa and 170 kDa
• GenBank Accession Number: BC018718
• Gene Symbol: EHMT2
• Gene ID (NCBI): 10919
• RRID: AB_2882043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96KQ7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.