EIF2A/CDA02 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

EIF2A/CDA02 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G7H4

Cat no : 66482-1-PBS

Synonyms

CDA02, EIF 2A, EIF2A, EIF2A/CDA02, MST089, MSTP004, MSTP089

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 66482-1-Ig (same clone as 66482-1-PBS)

  WB result of EIF2A/CDA02 antibody (66482-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2A/CDA02 transfected HEK-293 cells. This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66482-1-Ig (same clone as 66482-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66482-1-Ig (EIF2A/CDA02 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 66482-1-Ig (same clone as 66482-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66482-1-Ig (EIF2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66482-1-Ig (same clone as 66482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66482-1-Ig (EIF2A antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66482-1-Ig (same clone as 66482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66482-1-Ig (EIF2A antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66482-1-Ig (same clone as 66482-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66482-1-Ig (EIF2A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66482-1-PBS targets EIF2A/CDA02 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF2A/CDA02 fusion protein Ag22041
• Full Name: eukaryotic translation initiation factor 2A, 65kDa
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC011885
• Gene Symbol: EIF2A
• Gene ID (NCBI): 83939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.