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  • KD/KO Validated

EIF2A/CDA02 Monoclonal antibody

EIF2A/CDA02 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rat and More (1)







Cat no : 66482-1-Ig


CDA02, EIF 2A, EIF2A, EIF2A/CDA02, MST089, MSTP004, MSTP089

Tested Applications

Positive WB detected inA431 cells, ROS1728 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66482-1-Ig targets EIF2A/CDA02 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF2A/CDA02 fusion protein Ag22041
Full Name eukaryotic translation initiation factor 2A, 65kDa
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC011885
Gene Symbol EIF2A
Gene ID (NCBI) 83939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.


Product Specific Protocols
WB protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
IHC protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
IF protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Pharm Biol

Cinobufagin induces acute promyelocytic leukaemia cell apoptosis and PML-RARA degradation in a caspase-dependent manner by inhibiting the β-catenin signalling pathway

Authors - Yaoyao Bian

Front Neurosci

Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease

Authors - Fahad Mubariz


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Dorota (Verified Customer) (04-10-2019)

Very good for western blotting.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:250 -1:1000
  • Cell Tissue Type: U2OS
EIF2A/CDA02 Antibody Western Blot, validation (1:250 -1:1000 dilution) in U2OS (Cat no:66482-1-Ig)