Tested Applications
| Positive WB detected in | A431 cells, ROS1728 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66482-1-Ig targets EIF2A/CDA02 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22041 Product name: Recombinant human EIF2A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 238-585 aa of BC011885 Sequence: IASTDVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPIYDVVWNSSSTEFCAVYGFMPAKATIFNLKCDPVFDFGTGPRNAAYYSPHGHILVLAGFGNLRGQMEVWDVKNYKLISKPVASDSTYFAWCPDGEHILTATCAPRLRVNNGYKIWHYTGSILHKYDVPSNAELWQVSWQPFLDGIFPAKTITYQAVPSEVPNEEPKVATAYRPPALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRKHEAKKAAKQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQLKEQAATGKQLEKNQLEKIQKETALLQELEDLKLGI Predict reactive species |
| Full Name | eukaryotic translation initiation factor 2A, 65kDa |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC011885 |
| Gene Symbol | EIF2A |
| Gene ID (NCBI) | 83939 |
| RRID | AB_2881848 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9BY44 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
| IHC protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
| WB protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF2A/CDA02 Monoclonal antibody (1G7H4), catalog number 66482-1-Ig, has 5 total citations. These appear in Neuron, Pharmaceutical Biology, Scientific Reports, Frontiers in Physiology, and Frontiers in Neuroscience. The associated research fields span neurodegenerative diseases (ALS, Parkinson's disease), oncology (acute promyelocytic leukemia, breast cancer), and metabolic disorders (type 2 diabetes-related skeletal muscle atrophy). All citations used the antibody for Western blot applications across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Neuron Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes. | ||
Pharm Biol Cinobufagin induces acute promyelocytic leukaemia cell apoptosis and PML-RARA degradation in a caspase-dependent manner by inhibiting the β-catenin signalling pathway | ||
Sci Rep Induction of paraptotic cell death in breast cancer cells by an iron chelator involved activation of UPR/MAPK pathway and inactivation of PI3K/AKT/mTOR. | ||
Front Neurosci Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The reviewer used it for Western blotting on U2OS cells at dilutions ranging from 1:250 to 1:1000, and described the antibody as "very good for western blotting." Overall, the feedback indicates reliable performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Dorota (Verified Customer) (04-10-2019) | Very good for western blotting.
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