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EIF2A/CDA02 Monoclonal antibody

EIF2A/CDA02 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1G7H4

Cat no : 66482-1-Ig

Synonyms

CDA02, EIF 2A, EIF2A, EIF2A/CDA02, MST089, MSTP004, MSTP089



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells, ROS1728 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66482-1-Ig targets EIF2A/CDA02 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF2A/CDA02 fusion protein Ag22041
Full Name eukaryotic translation initiation factor 2A, 65kDa
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011885
Gene symbol EIF2A
Gene ID (NCBI) 83939
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.

Protocols

Product Specific Protocols
WB protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
IHC protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
IF protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Dorota (Verified Customer) (04-10-2019)

Very good for western blotting.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:250 -1:1000
  • Cell Tissue Type: U2OS
EIF2A/CDA02 Antibody Western Blot, validation (1:250 -1:1000 dilution) in U2OS (Cat no:66482-1-Ig)