EIF2AK1 Polyclonal antibody, PBS Only

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• EIF2AK1 Polyclonal antibody, PBS Only (20499-1-PBS)

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  WB analysis of HEK-293 using 20499-1-AP (same clone as 20499-1-PBS)

  WB result of EIF2AK1 antibody (20499-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

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  WB analysis using 20499-1-AP (same clone as 20499-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 20499-1-AP (same clone as 20499-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20499-1-AP (same clone as 20499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20499-1-AP (same clone as 20499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20499-1-AP (same clone as 20499-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2AK1 antibody (20499-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

EIF2AK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 20499-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:2.7.11.1, Eukaryotic translation initiation factor 2-alpha kinase 1, HCR, Heme controlled repressor, Heme regulated inhibitor

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Product Information

20499-1-PBS targets EIF2AK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14236

  Product name: Recombinant human EIF2AK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 281-630 aa of BC006524

  Sequence: FAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG

• Full Name: eukaryotic translation initiation factor 2-alpha kinase 1
• Calculated Molecular Weight: 630 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC006524
• Gene Symbol: EIF2AK1
• Gene ID (NCBI): 27102
• RRID: AB_10697665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BQI3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions.