Tested Applications
| Positive WB detected in | MCF-7 cells, K-562 cells, HeLa cells, HL-60 cells, mouse skeletal muscle tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11332-1-AP targets EIF2B4 in WB, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1875 Product name: Recombinant human EIF2B4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-341 aa of BC001870 Sequence: MAAVAVAVREDSGSGMKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPETGSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKGEQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSL Predict reactive species |
| Full Name | eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa |
| Calculated Molecular Weight | 523 aa, 58 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC001870 |
| Gene Symbol | EIF2B4 |
| Gene ID (NCBI) | 8890 |
| RRID | AB_2277640 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UI10 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| WB protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-eIF2B antibody (Cat# 11332-1-AP) has 12 citations published in journals including Nature Communications, Molecular Cell, ACS Central Science, EMBO Journal, Cell Oncology, FASEB Journal, Journal of Biological Chemistry, Biochemical Journal, PLoS ONE, and eLife. Associated research fields encompass integrated stress response regulation, eIF2B complex structure and stoichiometry, mitophagy, colorectal cancer, hepatocellular carcinoma, leukemogenesis, KRas signaling, and Vanishing White Matter Disease.
| Species | Application | Title |
|---|---|---|
Mol Cell The HRI branch of the integrated stress response selectively triggers mitophagy
| ||
ACS Cent Sci Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b. | ||
EMBO J Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer
| ||
Cell Oncol (Dordr) EIF2B4 promotes hepatocellular carcinoma progression and immune evasion by driving STAT3 translation via a GEF-dependent mechanism.
| ||
FASEB J Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation. |
