EIF2B4 Polyclonal antibody

EIF2B4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11332-1-AP

Synonyms

EIF 2B, EIF2B, EIF2B4, EIF2BD, EIF2Bdelta, eIF2Bδ

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Validation Data Gallery

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  WB analysis using 11332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11332-1-AP (EIF2B4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11332-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11332-1-AP (EIF2B4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 11332-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 11332-1-AP (EIF2B4 Antibody) at dilution of 1:50.

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  IF Staining of MCF-7 using 11332-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 11332-1-AP (EIF2B4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, K-562 cells, HeLa cells, HL-60 cells, mouse skeletal muscle tissue
• Positive IHC detected in: human breast cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below

Product Information

11332-1-AP targets EIF2B4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF2B4 fusion protein Ag1875
• Full Name: eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa
• Calculated Molecular Weight: 523 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC001870
• Gene Symbol: EIF2B4
• Gene ID (NCBI): 8890
• RRID: AB_2277640
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Sugar phosphate activation of the stress sensor eIF2B.; Authors - Qi Hao
human	WB	Elife; Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.; Authors - Carmela Sidrauski
	WB	Elife; The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.; Authors - Yao Liang Wong
human	WB	ACS Cent Sci; Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b.; Authors - Xiaoyu Zhang
human	WB	Elife; eIF2B conformation and assembly state regulate the integrated stress response.; Authors - Michael Schoof
human, mouse	WB	FASEB J; Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.; Authors - Noel C Wortham