- Featured Product
- KD/KO Validated
EIF2S1/EIF2A Polyclonal antibody
EIF2S1/EIF2A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11170-1-AP
|Positive WB detected in
|HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells
|Positive IP detected in
|Positive IHC detected in
|human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11170-1-AP targets EIF2S1/EIF2A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, pig
|Host / Isotype
|Rabbit / IgG
|EIF2S1/EIF2A fusion protein Ag1645
|eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.
|Product Specific Protocols
|WB protocol for EIF2S1/EIF2A antibody 11170-1-AP
|IHC protocol for EIF2S1/EIF2A antibody 11170-1-AP
|IF protocol for EIF2S1/EIF2A antibody 11170-1-AP
|IP protocol for EIF2S1/EIF2A antibody 11170-1-AP
|Click here to view our Standard Protocols
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