EIF2S1/EIF2A Polyclonal antibody

EIF2S1/EIF2A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

43

Cat no : 11170-1-AP

Synonyms

EIF 2, eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1/EIF2A, eIF2α

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Validation Data Gallery

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  WB analysis using 11170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11170-1-AP (EIF2S1/EIF2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11170-1-AP (EIF2S1/EIF2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11170-1-AP

  IP result of anti-EIF2S1/EIF2A(IP:11170-1-AP, 4ug; Detection:11170-1-AP 1:8000) with HepG2 cells lysate 1640 ug.

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  IHC staining of human colon cancer using 11170-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11170-1-AP (EIF2S1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11170-1-AP

  Immunofluorescent analysis of HepG2 cells, using EIF2S1 antibody 11170-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HeLa using 11170-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EIF2S1/EIF2A (11170-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:2000-1:16000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 42 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

11170-1-AP targets EIF2S1/EIF2A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF2S1/EIF2A fusion protein Ag1645
• Full Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Calculated molecular weight: 36 kDa
• Observed molecular weight: 36 kDa
• GenBank accession number: BC002513
• Gene symbol: EIF2S1
• Gene ID (NCBI): 1965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.

Publications

Species	Application	Title
human	WB	Mol Cancer; Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation.; Authors - Mengyan Xie
human	WB	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
pig	WB	Autophagy; Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.; Authors - Sheng-Li Ming
pig	WB	Autophagy; STING1 is essential for an RNA-virus triggered autophagy.; Authors - Rui Zhang
mouse	WB	J Hazard Mater; Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells.; Authors - Qiushuang Zhu
human	WB	Biochem Pharmacol; The organic arsenical-derived thioredoxin and glutathione system inhibitor ACZ2 induces apoptosis and autophagy in gastric cancer via ROS-dependent ER stress; Authors - Yi Li