EIF2S1/EIF2A Polyclonal antibody

EIF2S1/EIF2A Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11170-1-AP

Synonyms

EIF 2, eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1/EIF2A



Tested Applications

Positive WB detected inhuman stomach tissue, human placenta tissue, human skeletal muscle tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11170-1-AP targets EIF2S1/EIF2A in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2S1/EIF2A fusion protein Ag1645
Full Name eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002513
Gene symbol EIF2S1
Gene ID (NCBI) 1965
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Protocols

Product Specific Protocols
WB protocol for EIF2S1/EIF2A antibody 11170-1-APDownload protocol
IHC protocol for EIF2S1/EIF2A antibody 11170-1-APDownload protocol
IF protocol for EIF2S1/EIF2A antibody 11170-1-APDownload protocol
IP protocol for EIF2S1/EIF2A antibody 11170-1-APDownload protocol
FC protocol for EIF2S1/EIF2A antibody 11170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Reactive oxygen species mediate anlotinib-induced apoptosis via activation of endoplasmic reticulum stress in pancreatic cancer.

Authors - Liguo Yang
ratWB

Acta Biochim Biophys Sin (Shanghai)

Exogenous hydrogen sulfide inhibits apoptosis by regulating endoplasmic reticulum stress-autophagy axis and improves myocardial reconstruction after acute myocardial infarction.

Authors - Yaling Li
ratWB

Phytother Res

Potent Effects of the Total Saponins from Dioscorea nipponica Makino Against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats.

Authors - Hao Yu
humanWB

Environ Toxicol

ROS-mediated PERK-eIF2α-ATF4 pathway plays an important role in arsenite-induced L-02 cells apoptosis via regulating CHOP-DR5 signaling.

Authors - Chunyan Liu
mouseIHC

Diabetes Metab Syndr Obes

The Effects of Puerarin on Autophagy Through Regulating of the PERK/eIF2α/ATF4 Signaling Pathway Influences Renal Function in Diabetic Nephropathy.

Authors - Xiaohui Xu
mouseWB

Exp Eye Res

PMID: 31801685

Authors - Wenqin Xu