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EIF3K Polyclonal antibody

EIF3K Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

ARG134, eIF 3 p25, eIF 3 p28, EIF3 p28, EIF3K, EIF3S12, HSPC029, M9, MSTP001, PLAC 24, PLAC24, PRO1474, PTD001

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

-/ -

Freight/Packing: -

Quantity

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What's the best dilution for EIF3K in ?

Tested Applications

Positive WB detected inHEK-293 cells, NCI-H1299 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10640-1-AP targets EIF3K in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1000

Product name: Recombinant human EIF3K protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-218 aa of BC007559

Sequence: MAMFEQMRANVGKLLKGIDRYNPENLATLERYVETQAKENAYDLEANLAVLKLYQFNPAFFQTTVTAQILLKALTNLPHTDFTLCKCMIDQAHQEERPIRQILYLGDLLETCHFQAFWQALDENMDLLEGITGFEDSVRKFICHVVGITYQHIDRWLLAEMLGDLSDSQLKVWMSKYGWSADESGQIFICSQEESIKPKNIVEKIDFDSVSSIMASSQ

Predict reactive species
Full Name eukaryotic translation initiation factor 3, subunit K
Calculated Molecular Weight 28 kDa
GenBank Accession NumberBC007559
Gene Symbol EIF3K
Gene ID (NCBI) 27335
RRIDAB_2262100
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBQ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EIF3K antibody 10640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The EIF3K Polyclonal antibody (Cat# 10640-1-AP) has 2 citations. One appears in Nature Communications (2020), studying a translational program that suppresses metabolism to shield the genome, and the other in Environmental Toxicology (2021), investigating how SMAR1 attenuates osteosarcoma stemness by suppressing ABCG2 transcriptional activity. Both citations used the antibody for Western Blot in human samples, covering research fields in molecular biology and cancer biology.

SpeciesApplicationTitle
humanWB

Nat Commun

A translational program that suppresses metabolism to shield the genome.

Authors - Nathan C Balukoff
humanWB

Environ Toxicol

SMAR1 attenuates the stemness of osteosarcoma cells via through suppressing ABCG2 transcriptional activity.

Authors - Hongyu Xu
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