Tested Applications
| Positive WB detected in | HEK-293 cells, NCI-H1299 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10640-1-AP targets EIF3K in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1000 Product name: Recombinant human EIF3K protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-218 aa of BC007559 Sequence: MAMFEQMRANVGKLLKGIDRYNPENLATLERYVETQAKENAYDLEANLAVLKLYQFNPAFFQTTVTAQILLKALTNLPHTDFTLCKCMIDQAHQEERPIRQILYLGDLLETCHFQAFWQALDENMDLLEGITGFEDSVRKFICHVVGITYQHIDRWLLAEMLGDLSDSQLKVWMSKYGWSADESGQIFICSQEESIKPKNIVEKIDFDSVSSIMASSQ Predict reactive species |
| Full Name | eukaryotic translation initiation factor 3, subunit K |
| Calculated Molecular Weight | 28 kDa |
| GenBank Accession Number | BC007559 |
| Gene Symbol | EIF3K |
| Gene ID (NCBI) | 27335 |
| RRID | AB_2262100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBQ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF3K antibody 10640-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF3K Polyclonal antibody (Cat# 10640-1-AP) has 2 citations. One appears in Nature Communications (2020), studying a translational program that suppresses metabolism to shield the genome, and the other in Environmental Toxicology (2021), investigating how SMAR1 attenuates osteosarcoma stemness by suppressing ABCG2 transcriptional activity. Both citations used the antibody for Western Blot in human samples, covering research fields in molecular biology and cancer biology.
| Species | Application | Title |
|---|---|---|
Environ Toxicol SMAR1 attenuates the stemness of osteosarcoma cells via through suppressing ABCG2 transcriptional activity. |

