EIF3M Monoclonal antibody

EIF3M Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2A12B2

Cat no : 66025-1-Ig

Synonyms

B5, EIF3M, Fetal lung protein B5, GA17, hfl B5, HFLB5, PCID1, PNAS 125



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66025-1-Ig is EIF3M Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EIF3M fusion protein Ag17965
Full Name eukaryotic translation initiation factor 3, subunit M
Calculated molecular weight 374 aa, 43 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberBC019103
Gene symbol EIF3M
Gene ID (NCBI) 10480
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.

Protocols

Product Specific Protocols
WB protocol for EIF3M antibody 66025-1-IgDownload protocol
IHC protocol for EIF3M antibody 66025-1-IgDownload protocol
IF protocol for EIF3M antibody 66025-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols