EIF4B Polyclonal antibody

EIF4B Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17917-1-AP


EIF 4B, EIF4B, PRO1843

Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IF detected inMCF-7 cells, sodium arsenite treated HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17917-1-AP targets EIF4B in WB,IF,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4B fusion protein Ag12325
Full Name eukaryotic translation initiation factor 4B
Calculated Molecular Weight 611 aa, 69 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC073154
Gene Symbol EIF4B
Gene ID (NCBI) 1975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).


Product Specific Protocols
WB protocol for EIF4B antibody 17917-1-APDownload protocol
IF protocol for EIF4B antibody 17917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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