EIF4E2 Polyclonal antibody

EIF4E2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12227-1-AP

Synonyms

4E LP, 4EHP, eIF 4E type 2, eIF4E like protein 4E LP, eIF4E type 2, EIF4E2, EIF4EL3, IF4e, mRNA cap binding protein 4EHP

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Validation Data Gallery

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  WB analysis of HeLa using 12227-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12227-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12227-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12227-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12227-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 12227-1-AP

  IP result of anti-EIF4E2 (IP:12227-1-AP, 4ug; Detection:12227-1-AP 1:500) with MCF-7 cells lysate 1280ug.

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  IHC staining of human breast cancer using 12227-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 12227-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 12227-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 12227-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, mouse kidney tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Product Information

12227-1-AP targets EIF4E2 in WB, RIP, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF4E2 fusion protein Ag2864
• Full Name: eukaryotic translation initiation factor 4E family member 2
• Calculated Molecular Weight: 245 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC021690
• Gene Symbol: EIF4E2
• Gene ID (NCBI): 9470
• RRID: AB_10642945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,RIP	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
human	WB	iScience; The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.; Authors - Limei Zou
human	WB,IHC	Eur J Med Res; N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade glioma; Authors - Aierpati Maimaiti
human	WB	Nat Struct Mol Biol; Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancer; Authors - Yoichiro Sugimoto