EIF5A2 Monoclonal antibody

EIF5A2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F9B2

Cat no : 67907-1-Ig

Synonyms

eIF 5A 2, EIF 5A2, EIF5A2, eIF5AII

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Validation Data Gallery

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  WB analysis using 67907-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67907-1-Ig (EIF5A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of human lung cancer using 67907-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67907-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67907-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67907-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67907-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using EIF5A2 antibody (67907-1-Ig, Clone: 1F9B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
• Positive IHC detected in: human lung cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67907-1-Ig targets EIF5A2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF5A2 fusion protein Ag10852
• Full Name: eukaryotic translation initiation factor 5A2
• Calculated Molecular Weight: 153 aa, 17 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC036072
• Gene Symbol: EIF5A2
• Gene ID (NCBI): 56648
• RRID: AB_2918663
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor 5A (EIF5A), a conserved lysine to hypusine, is strictly indispensable for the survival of eukaryotic cells. There are two EIF5A isoforms in humans: EIF5A1 and EIF5A2. EIF5A2 has been reported to be overexpressed in gallbladder cancer, upper urinary tract urothelial carcinoma, gastric cancer, prostate cancer, nasopharyngeal carcinoma, and cervical cancer. EIF5A2 upregulation in cancer tissues is associated with poor prognosis in these patients.