ELAVL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ELAVL2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 14008-1-AP

Synonyms

HUB, Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2

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Validation Data Gallery

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  WB analysis using 14008-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14008-1-AP (ELAVL2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 14008-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14008-1-AP (ELAVL2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse cerebellum using 14008-1-AP

  IP result of anti-ELAVL2 (IP:14008-1-AP, 4ug; Detection:14008-1-AP 1:30000) with mouse cerebellum tissue lysate 3000 ug.

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  IHC staining of human brain using 14008-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14008-1-AP (ELAVL2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 14008-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14008-1-AP (ELAVL2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse cerebellum tissue
• Positive IP detected in: mouse cerebellum tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• RIP: See 3 publications below

Product Information

14008-1-AP targets ELAVL2 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ELAVL2 fusion protein Ag5105
• Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC030692
• Gene Symbol: ELAVL2
• Gene ID (NCBI): 1993
• RRID: AB_2096356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].

Publications

Species	Application	Title
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
mouse	IP	Sci Adv; Mature mRNA processing that deletes 3' end sequences directs translational activation and embryonic development; Authors - Yuki Takada
human	WB,IF,RIP	Nucleic Acids Res; HuB and HuD repress telomerase activity by dissociating HuR from TERC.; Authors - Xiaolei Cheng ; KD Validated
mouse	RIP	EMBO Rep; ELAVL2-directed RNA regulatory network drives the formation of quiescent primordial follicles.; Authors - Yuzuru Kato
chicken	IF	Cell Rep; miR-302 Is Required for Timing of Neural Differentiation, Neural Tube Closure, and Embryonic Viability.; Authors - Ronald J Parchem
human	WB	Hum Mol Genet; ELAVL2-regulated transcriptional and splicing networks in human neurons link neurodevelopment and autism.; Authors - Stefano Berto ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jacopo (Verified Customer) (09-03-2019)

Very noce and specific bands in western blot. Also works for RIP

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioma stem cells