Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse cerebellum tissue |
| Positive IP detected in | mouse cerebellum tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14008-1-AP targets ELAVL2 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5105 Product name: Recombinant human ELAVL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 5-346 aa of BC030692 Sequence: LSNGPTCNNTANGPTTINNNCSSPVDSGNTEDSKTNLIVNYLPQNMTQEELKSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGISRGVGFIRFDKRIEAEEAIKGLNGQKPPGATEPITVKFANNPSQKTNQAILSQLYQSPNRRYPGPLAQQAQRFRFSPMTIDGMTSLAGINIPGHPGTGWCIFVYNLAPDADESILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKTHKA Predict reactive species |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B) |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC030692 |
| Gene Symbol | ELAVL2 |
| Gene ID (NCBI) | 1993 |
| RRID | AB_2096356 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12926 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ELAVL2 antibody 14008-1-AP | Download protocol |
| IP protocol for ELAVL2 antibody 14008-1-AP | Download protocol |
| WB protocol for ELAVL2 antibody 14008-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ELAVL2 Polyclonal antibody (Cat# 14008-1-AP) has accumulated 26 citations across 21 journals, including Molecular Cell, Nucleic Acids Research, Science Advances, Cell Reports, EMBO Journal, Journal of Neuroscience, Development, and Journal of Biological Chemistry. Associated research fields encompass neurodegeneration (Alzheimer's disease, ALS), RNA biology and alternative splicing, neurodevelopment, cancer, inflammation, and cardiovascular disease.
| Species | Application | Title |
|---|---|---|
Mol Neurodegener Increased expression of mesencephalic astrocyte-derived neurotrophic factor (MANF) contributes to synapse loss in Alzheimer's disease | ||
Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages | ||
Nucleic Acids Res HuB and HuD repress telomerase activity by dissociating HuR from TERC.
| ||
Sci Adv Mature mRNA processing that deletes 3' end sequences directs translational activation and embryonic development | ||
Cell Death Dis ApoM maintains cellular homeostasis between mitophagy and apoptosis by affecting the stability of Nnt mRNA through the Zic3-ApoM-Elavl2-Nnt axis during neural tube closure | ||
Cell Genom Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders |
Reviews
What customers say
The single review for 14008-1-AP is from Jacopo Zasso (2019), who rated it 4 out of 5 stars. Used at a 1:1000 dilution on glioma stem cells for Western Blot and RIP, they reported "very nice and specific bands in western blot" and noted it also works well for RIP. Overall, the reviewer found the antibody to be specific and reliable across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jacopo (Verified Customer) (09-03-2019) | Very noce and specific bands in western blot. Also works for RIP
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