ELAVL2 Monoclonal antibody

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• ELAVL2 Monoclonal antibody (67097-1-Ig)

ELAVL2 Monoclonal Antibody for WB, ELISA

Cat No. 67097-1-Ig Copied!

Clone No. 2D8C10

Publications (3)

Reviews (1)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF, IP, ELISA

Synonyms

2D8C10, ELAV2, ELAVL 2, ELAV-like neuronal protein 1, ELAV-like protein 2

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  WB analysis using 67097-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Neuro-2a using 67097-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of pig brain using 67097-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 67097-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67097-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 67097-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-12 using 67097-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SH-SY5Y using 67097-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Neuro-2a using 67097-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-12 using 67097-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, SH-SY5Y cells, PC-12 cells, Neuro-2a cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67097-1-Ig targets ELAVL2 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28310

  Product name: Recombinant human ELAVL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 5-346 aa of BC030692

  Sequence: LSNGPTCNNTANGPTTINNNCSSPVDSGNTEDSKTNLIVNYLPQNMTQEELKSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGISRGVGFIRFDKRIEAEEAIKGLNGQKPPGATEPITVKFANNPSQKTNQAILSQLYQSPNRRYPGPLAQQAQRFRFSPMTIDGMTSLAGINIPGHPGTGWCIFVYNLAPDADESILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKTHKA

• Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC030692
• Gene Symbol: ELAVL2
• Gene ID (NCBI): 1993
• RRID: AB_2882402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q12926
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].

Publications

What published studies show

The ELAVL2 Monoclonal antibody (2D8C10), catalog number 67097-1-Ig, has 3 citations. These appear in Molecular Neurodegeneration, Science Advances, and Acta Neuropathologica. The associated research fields include Alzheimer's disease (synapse loss), neuronal granule biology and nervous system maturity, and amyotrophic lateral sclerosis (ALS). Applications cited are IP, WB, and IF, with species including mouse and human.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IP	Mol Neurodegener; Increased expression of mesencephalic astrocyte-derived neurotrophic factor (MANF) contributes to synapse loss in Alzheimer's disease; Authors - Yiran Zhang
mouse	WB	Sci Adv; The long noncoding RNA mimi scaffolds neuronal granules to maintain nervous system maturity; Authors - Dominika Grzejda
human	IF	Acta Neuropathol; Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.; Authors - Sandra Diaz-Garcia

Reviews

1.0 out of 5

1 verified customer reviews

What customers say

One user review was found for this product. Petr Kalab rated it 1 out of 5 stars on July 19, 2023, after using it for Western Blot at a 1/1000 dilution (lot 10009461). No written comments were provided with the review.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Petr (Verified Customer) (07-19-2023)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000