ELAVL2 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 67097-1-Ig (same clone as 67097-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 67097-1-Ig (same clone as 67097-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67097-1-Ig (same clone as 67097-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67097-1-Ig (same clone as 67097-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67097-1-Ig (same clone as 67097-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67097-1-Ig (same clone as 67097-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 67097-1-Ig (same clone as 67097-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 67097-1-Ig (same clone as 67097-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67097-1-Ig (same clone as 67097-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67097-1-Ig (same clone as 67097-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50972-1

  Cytometric bead array standard curve of MP50972-1, ELAVL2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67097-1-PBS. Detection antibody: 67097-2-PBS. Standard:Ag28310. Range: 0.391-100 ng/mL.

ELAVL2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 67097-1-PBS

Clone No.2D8C10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2, ELAV2

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Product Information

67097-1-PBS targets ELAVL2 as part of a matched antibody pair:

MP50972-1: 67097-1-PBS capture and 67097-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28310

  Product name: Recombinant human ELAVL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 5-346 aa of BC030692

  Sequence: LSNGPTCNNTANGPTTINNNCSSPVDSGNTEDSKTNLIVNYLPQNMTQEELKSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGISRGVGFIRFDKRIEAEEAIKGLNGQKPPGATEPITVKFANNPSQKTNQAILSQLYQSPNRRYPGPLAQQAQRFRFSPMTIDGMTSLAGINIPGHPGTGWCIFVYNLAPDADESILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKTHKA

• Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC030692
• Gene Symbol: ELAVL2
• Gene ID (NCBI): 1993
• RRID: AB_2882402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q12926
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].