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  • KD/KO Validated

ELF2 Polyclonal antibody

ELF2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12499-1-AP

Synonyms

E74 like factor 2, ELF2, ELF2A, EU32, NERF, NERF 1A, NERF 1a,b, NERF 1B, NERF 2, New ETS related factor



Tested Applications

Positive WB detected inA2780 cells, human brain tissue, human liver tissue
Positive IP detected inA2780 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12499-1-AP targets ELF2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELF2 fusion protein Ag3170
Full Name E74-like factor 2 (ets domain transcription factor)
Calculated Molecular Weight 533 aa, 57 kDa
Observed Molecular Weight 63-64 kDa, 56-57 kDa
GenBank Accession NumberBC034951
Gene Symbol ELF2
Gene ID (NCBI) 1998
RRIDAB_10642956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ets-1 is the prototype member of a family of genes firstly identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family have varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. ELF2 belongs to the Ets-1 family. ELF2 exists several isoforms and the range of molucular weight of isoform is about 54-66 kDa.

Protocols

Product Specific Protocols
WB protocol for ELF2 antibody 12499-1-APDownload protocol
IP protocol for ELF2 antibody 12499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
goatWB

Front Cell Dev Biol

A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development.

Authors - Yue Zhang
  • KD Validated
humanWB

Cell Biol Int

Inhibition of the miR-193b-3p protects against oxidized low-density lipoprotein-induced HUVECs injury by upregulating ALDH2.

Authors - Yunpeng Bai
mouseWB

Ren Fail

ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyte.

Authors - Shizhu Yuan