ELF2 Polyclonal antibody

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• KD/KO Validated

ELF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12499-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Synonyms

E74 like factor 2, E74-like factor 2, ELF2A, ETS-related transcription factor Elf-2, EU32

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  WB analysis using 12499-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 12499-1-AP

  WB result of ELF2 antibody (12499-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELF2 transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse colon using 12499-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12499-1-AP (ELF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 12499-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12499-1-AP (ELF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 12499-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12499-1-AP (ELF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 12499-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ELF2 antibody (12499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: rat colon tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12499-1-AP targets ELF2 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3170

  Product name: Recombinant human ELF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-353 aa of BC034951

  Sequence: MATSLHEGPTNQLDLLIRAVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPVEVFVPPCVSTPEFIHAAMRPDVITETVVEVSTEESEPMDTSPIPTSPDSHEPMKKKKVGRKPKTQQSPISNGSPELGIKKKPREGKGNTTYLWEFLLDLLQDKNTCPRYIKWTQREKGIFKLVDSKAVSKLWGKHKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKDMPKNIVVIDDDKSETCNEDLAGTTDEKSLERVSLSAESLLKAASSVRSGKNSSPINCSRAEKGVARVVNITSPGHDASSRSPTTTASVSATAAPRTVRVAMQVPVVMTSLGQKISTVAVQSV

• Full Name: E74-like factor 2 (ets domain transcription factor)
• Calculated Molecular Weight: 533 aa, 57 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC034951
• Gene Symbol: ELF2
• Gene ID (NCBI): 1998
• RRID: AB_10642956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15723
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ets-1 is the prototype member of a family of genes firstly identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family have varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. ELF2 belongs to the Ets-1 family.

Publications

What published studies show

The ELF2 Polyclonal antibody (Cat# 12499-1-AP) has 8 citations published in Nature Communications, Molecular Cell, Cell Death & Disease, Frontiers in Cell and Developmental Biology, Balkan Medical Journal, Renal Failure, Cell Biology International, and medRxiv. Research fields span pancreatic cancer genetics, p53 binding, retinoblastoma, mammary gland development, ER stress–induced apoptosis, diabetic nephropathy, and endothelial cell injury.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	ChIP	Nat Commun; Allelic effects on KLHL17 expression underlie a pancreatic cancer genome-wide association signal at chr1p36.33; Authors - Katelyn E Connelly
human	IF	Mol Cell; Targeted citrullination enables p53 binding to non-canonical sites.; Authors - Alexandra Indeglia
human	WB	Cell Death Dis; Loss of ELF2 drives topotecan resistance in retinoblastoma revealed by genome-wide CRISPR-Cas9 screening.; Authors - Jingyi Jiang ; KO Validated
goat	WB	Front Cell Dev Biol; A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development.; Authors - Yue Zhang ; KD Validated
	WB	Balkan Med J; Quercetin Alleviates Hyperglycemic-Generated Endoplasmic Reticulum Stress-Contacted Apoptosis of Rat Nucleus Pulposus Cells; Authors - Zhen Yu
mouse	WB	Ren Fail; ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyte.; Authors - Shizhu Yuan