ELK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ELK1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 27420-1-AP

Synonyms

ELK1

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Validation Data Gallery

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  WB analysis using 27420-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27420-1-AP (ELK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27420-1-AP

  WB result of ELK1 antibody (27420-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELK1 transfected HepG2 cells.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells,

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

27420-1-AP targets ELK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ELK1 fusion protein Ag26683
• Full Name: ELK1, member of ETS oncogene family
• Calculated molecular weight: 45 kDa
• Observed molecular weight: 62 kDa
• GenBank accession number: BC056150
• Gene symbol: ELK1
• Gene ID (NCBI): 2002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Oncol; tRNA-Derived Fragment tRF-Glu-TTC-027 Regulates the Progression of Gastric Carcinoma via MAPK Signaling Pathway; Authors - Weiguo Xu
rat	IHC	J Transl Med; The SETD8/ELK1/bach1 complex regulates hyperglycaemia-mediated EndMT in diabetic nephropathy.; Authors - Xue Li
human	WB	Biochem Biophys Res Commun; CircRNA circ-0038718 promotes hepatocellular carcinoma progression through sponging miR-139-3p.; Authors - Ranran Sun
human	WB	Genes Genomics; Tumor-suppressive miRNA-135a inhibits breast cancer cell proliferation by targeting ELK1 and ELK3 oncogenes.; Authors - Akhlaq Ahmad ; KD Validated