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  • KD/KO Validated

ELL Polyclonal antibody

ELL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 51044-1-AP

Synonyms

C19orf17, ELL, ELL1, MEN



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HL-60 cells, mouse testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51044-1-AP targets ELL in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELL fusion protein Ag0617
Full Name elongation factor RNA polymerase II
Calculated Molecular Weight 500 aa, 68 kDa
Observed Molecular Weight 68-80 kDa
GenBank Accession NumberBC049195
Gene Symbol ELL
Gene ID (NCBI) 8178
RRIDAB_2099137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELL is an elongation factor that inrecase the catalytic rate of RNA polymerase II transcription via suppressing transient pausing by the polymerase at multiple sites along the DNA. Also it's the second elongation factor to be involved in oncogenesis which is a transcription factor regulated by the product of the von Hippel-Lindau tumor suppressor. There are two transcirpts variants in mRNA level, one is 4.4kb transcript and the other is 2.8kb transcript.

Protocols

Product Specific Protocols
WB protocol for ELL antibody 51044-1-APDownload protocol
IHC protocol for ELL antibody 51044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Nat Commun

Aberrant activation of non-coding RNA targets of transcriptional elongation complexes contributes to TDP-43 toxicity.

Authors - Chia-Yu Chung
  • KD Validated
humanWB

Nat Commun

ELL targets c-Myc for proteasomal degradation and suppresses tumour growth.

Authors - Yu Chen
mouseWB

Oncogene

The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia.

Authors - Hao Wang
humanWB

Prostate

ELL is an HIF-1alpha partner that regulates and responds to hypoxia response in PC3 cells.

Authors - Liu Lingqi L
  • KD Validated
humanWB

Mol Immunol

Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival.

Authors - Lou-Ella M M Alexander
  • KD Validated
mouseWB,IP

Cell

Inherited blood cancer predisposition through altered transcription elongation

Authors - Jiawei Zhao