ELL Polyclonal antibody

• Featured Product
• KD/KO Validated

ELL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 51044-1-AP

Synonyms

C19orf17, ELL, ELL1, MEN

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Validation Data Gallery

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  WB analysis of HeLa using 51044-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 51044-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 51044-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51044-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 51044-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 51044-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51044-1-AP (ELL antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HL-60 cells, mouse testis tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

51044-1-AP targets ELL in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ELL fusion protein Ag0617
• Full Name: elongation factor RNA polymerase II
• Calculated Molecular Weight: 500 aa, 68 kDa
• Observed Molecular Weight: 68-80 kDa
• GenBank Accession Number: BC049195
• Gene Symbol: ELL
• Gene ID (NCBI): 8178
• RRID: AB_2099137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELL is an elongation factor that inrecase the catalytic rate of RNA polymerase II transcription via suppressing transient pausing by the polymerase at multiple sites along the DNA. Also it's the second elongation factor to be involved in oncogenesis which is a transcription factor regulated by the product of the von Hippel-Lindau tumor suppressor. There are two transcirpts variants in mRNA level, one is 4.4kb transcript and the other is 2.8kb transcript.

Publications

Species	Application	Title
human	WB,CoIP	Nat Commun; Aberrant activation of non-coding RNA targets of transcriptional elongation complexes contributes to TDP-43 toxicity.; Authors - Chia-Yu Chung ; KD Validated
human	WB	Nat Commun; ELL targets c-Myc for proteasomal degradation and suppresses tumour growth.; Authors - Yu Chen
mouse	WB	Oncogene; The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia.; Authors - Hao Wang
human	WB	Prostate; ELL is an HIF-1alpha partner that regulates and responds to hypoxia response in PC3 cells.; Authors - Liu Lingqi L ; KD Validated
human	WB	Mol Immunol; Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival.; Authors - Lou-Ella M M Alexander ; KD Validated
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao