ELL Polyclonal antibody, PBS Only

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• ELL Polyclonal antibody, PBS Only (51044-1-PBS)

• 

  WB analysis of HeLa using 51044-1-AP (same clone as 51044-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 51044-1-AP (same clone as 51044-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 51044-1-AP (same clone as 51044-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 51044-1-AP (same clone as 51044-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 51044-1-AP (same clone as 51044-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 51044-1-AP (same clone as 51044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51044-1-AP (ELL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ELL Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 51044-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

C19orf17, Eleven-nineteen lysine-rich leukemia protein, ELL1, MEN, PPP1R68

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Product Information

51044-1-PBS targets ELL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0617

  Product name: Recombinant human ELL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-500 aa of BC049195

  Sequence: MAALKEDRSYGLSCGRVSDGSKVSVFHVKLTDSALRAFESYRARQDSVSLRPSIRFQGSQGHISIPQPDCPAEARTFSFYLSNIGRDNPQGSFDCIQQYVSSHGEVHLDCLGSIQDKITVCATDDSYQKARQSMAQAEEETRSRSAIVIKAGGRYLGKKVQFRKPAPGATDAVPSRKRATPINLASAIRKSGASAVSGGSGVSQRPFRDRVLHLLALRPYRKAELLLRLQKDGLTQADKDALDGLLQQVANMSAKDGTCTLQDCMYKDVQKDWPGYSEGDQQLLKRVLVRKLCQPQSTGSLLGDPAASSPPGERGRSASPPQKRLQPPDFIDPLANKKPRISHFTQRAQPAVNGKLGVPNGREALLPTPGPPASTDTLSSSTHLPPRLEPPRAHDPLADVSNDLGHSGRDCEHGEAAAPAPTVRLGLPLLTDCAQPSRPHGSPSRSKPKKKSKKHKDKERAAEDKPRAQLPDCAPATHATPGAPADTPGLNGTCSVSSVP

• Full Name: elongation factor RNA polymerase II
• Calculated Molecular Weight: 500 aa, 68 kDa
• Observed Molecular Weight: 68-80 kDa
• GenBank Accession Number: BC049195
• Gene Symbol: ELL
• Gene ID (NCBI): 8178
• RRID: AB_2099137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55199
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ELL is an elongation factor that inrecase the catalytic rate of RNA polymerase II transcription via suppressing transient pausing by the polymerase at multiple sites along the DNA. Also it's the second elongation factor to be involved in oncogenesis which is a transcription factor regulated by the product of the von Hippel-Lindau tumor suppressor. There are two transcirpts variants in mRNA level, one is 4.4kb transcript and the other is 2.8kb transcript.