ELMO1 Polyclonal antibody, PBS Only

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• ELMO1 Polyclonal antibody, PBS Only (26974-1-PBS)

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  WB analysis using 26974-1-AP (same clone as 26974-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26974-1-AP (ELMO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 26974-1-AP (same clone as 26974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 26974-1-AP (same clone as 26974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 26974-1-AP (same clone as 26974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 26974-1-AP (same clone as 26974-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ELMO1 antibody (26974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 26974-1-AP (same clone as 26974-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ELMO1 antibody (26974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

ELMO1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 26974-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ELMO 1, engulfment and cell motility 1

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Product Information

26974-1-PBS targets ELMO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25635

  Product name: Recombinant human ELMO1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 76-158 aa of BC114341

  Sequence: RLTTSPAQNAQQLHERIQSSSMDAKLEALKDLASLSRDVTFAQEFINLDGISLLTQMVESGTERYQKLQKIMKPCFGDMLSFT

• Full Name: engulfment and cell motility 1
• Observed Molecular Weight: 80~84 kDa
• GenBank Accession Number: BC114341
• Gene Symbol: ELMO1
• Gene ID (NCBI): 9844
• RRID: AB_3742207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92556
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ELMO1 (Engulfment and cell motility protein 1) is a cytoplasmic adapter protein that physically associates with members of the Dock-A family of Rac-GEFs, of which Dock1 and Dock2 are the best characterized (PMID: 24821968). ELMO1 is expressed in platelets and negatively regulates integrin-mediated platelet spreading. Upregulated ELMO1 expression is associated with a poor prognosis in hepatocellular carcinoma (HCC), as well as increased cell growth, invasion, migration, angiogenesis and EMT in vitro and in vivo (PMID: 31324602).