ELOVL4 Polyclonal antibody

ELOVL4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 55023-1-AP

Synonyms

STGD2, ELOVL fatty acid elongase 4, ELOVL FA elongase 4, EC:2.3.1.199, ADMD

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Validation Data Gallery

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  WB analysis of Y79 using 55023-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 55023-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55023-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 55023-1-AP (ELOVL4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 55023-1-AP (ELOVL4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 55023-1-AP

  Immunofluorescent analysis of A431 cells using 55023-1-AP (ELOVL4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Y79 cells, A431 cells, mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human small intestine tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below

Product Information

55023-1-AP targets ELOVL4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: NM_022726
• Gene Symbol: ELOVL4
• Gene ID (NCBI): 6785
• RRID: AB_10859792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELOVL4, also named as ADMD, STGD2 and STGD3, belongs to the ELO family. It is a component of the fatty acid elongation system. Elovl4 is involved in the biosynthesis of very long chain fatty acids. It seems to represent a photoreceptor-specific component of the fatty acid elongation system residing on the endoplasmic reticulum. ELOVL4 may be implicated in docosahexaenoic acid (DHA) biosynthesis, which requires dietary consumption of the essential alpha-linolenic acid and a subsequent series of three elongation steps. Defects in ELOVL4 are the cause of Stargardt disease type 3 (STGD3). The antibody is specific to ELOVL4.

Publications

Species	Application	Title
human	WB	Ocul Surf; PPARγ regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC).; Authors - Sun Woong Kim
mouse	IHC	Ocul Surf; Ocular surface alkali injury damages meibomian glands in mice.; Authors - Shin Mizoguchi
mouse	WB	Int J Mol Sci; In Human and Mouse Spino-Cerebellar Tissue, Ataxin-2 Expansion Affects Ceramide-Sphingomyelin Metabolism.; Authors - Nesli-Ece Sen
rat	WB	Invest Ophthalmol Vis Sci; Isotretinoin Impairs the Secretory Function of Meibomian Gland Via the PPARγ Signaling Pathway.; Authors - Peng Zhang
human	WB	Lipids; Phytosphingosine Increases Biosynthesis of Phytoceramide by Uniquely Stimulating the Expression of Dihydroceramide C4-desaturase (DES2) in Cultured Human Keratinocytes.; Authors - Hyun Kyung Choi
mouse	IHC	Ocul Surf; Anomaly of cornea and ocular adnexa in spinster homolog 2 (Spns2) knockout mice; Authors - Shingo Yasuda