ELOVL4 Polyclonal antibody, PBS Only

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  WB analysis using 55023-1-AP (same clone as 55023-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Y79 using 55023-1-AP (same clone as 55023-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 55023-1-AP (same clone as 55023-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 55023-1-AP (same clone as 55023-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 55023-1-AP (same clone as 55023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 55023-1-AP (ELOVL4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 55023-1-AP (same clone as 55023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 55023-1-AP (ELOVL4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 55023-1-AP (same clone as 55023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 55023-1-AP (same clone as 55023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 55023-1-AP (same clone as 55023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 55023-1-AP (same clone as 55023-1-PBS)

  Immunofluorescent analysis of A431 cells using 55023-1-AP (ELOVL4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ELOVL4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 55023-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

STGD2, ELOVL fatty acid elongase 4, ELOVL FA elongase 4, EC:2.3.1.199, ADMD

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Product Information

55023-1-PBS targets ELOVL4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: NM_022726
• Gene Symbol: ELOVL4
• Gene ID (NCBI): 6785
• RRID: AB_10859792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9GZR5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ELOVL4, also named as ADMD, STGD2 and STGD3, belongs to the ELO family. It is a component of the fatty acid elongation system. Elovl4 is involved in the biosynthesis of very long chain fatty acids. It seems to represent a photoreceptor-specific component of the fatty acid elongation system residing on the endoplasmic reticulum. ELOVL4 may be implicated in docosahexaenoic acid (DHA) biosynthesis, which requires dietary consumption of the essential alpha-linolenic acid and a subsequent series of three elongation steps. Defects in ELOVL4 are the cause of Stargardt disease type 3 (STGD3). The antibody is specific to ELOVL4.