Tested Applications
| Positive WB detected in | HeLa cells, mouse skeletal muscle tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13946-1-AP targets EMB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5031 Product name: Recombinant human EMB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-253 aa of BC059398 Sequence: MRALPGLLEARARTPRLLLLQCLLAAARPSSADGSAPDSPFTSPPLREEIMANNFSLESHNISLTEHSSMPVEKNITLERPSNVNLTCQFTTSGDLNAVNVTWKKDGEQLENNYLVSATGSTLYTQYRFTIINSKQMGSYSCFFREEKEQRGTFNFKVPELHGKNKPLISYVGDSTVLTCKCQNCFPLNWTWYSSNGSVKVPVGVQMNKYVINGTYANETKLKITQLLEEDGESYWCRALFQLGESEEHIELV Predict reactive species |
| Full Name | embigin homolog (mouse) |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC059398 |
| Gene Symbol | EMB |
| Gene ID (NCBI) | 133418 |
| RRID | AB_2877993 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PCB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycosylated full-length protein with an molecular mass of 70 kDa.(PMID: 19164284)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EMB antibody 13946-1-AP | Download protocol |
| WB protocol for EMB antibody 13946-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One reviewer (Boyan Zhang, Oct 2021) used this antibody for Western Blot and reported a calculated size of 37 kD, but observed major bands at 65 and 55 kD plus a very weak band around 45 kD. All detected bands were PNGaseF insensitive, suggesting they were not glycosylated forms of the target protein. The reviewer rated the product 1 out of 5, indicating the observed bands did not match the expected molecular weight.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (10-04-2021) | calculated size 37 kd. This antibody recognized major bands at 65, 55 kd, and a very weak band ~45 kd. All of them were PNGaseF insensitive, suggesting they were not glycosylated form of this protein.
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