Emerin Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82888-3-RR (same clone as 82888-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82888-3-RR (Emerin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82888-3-RR (same clone as 82888-3-PBS)

  WB result of Emerin antibody (82888-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Emerin transfected HeLa cells. This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 82888-3-RR (same clone as 82888-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82888-3-RR (Emerin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82888-3-RR (same clone as 82888-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug EMD Recombinant antibody (82888-3-RR, Clone:230089B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82888-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82888-3-RR against Human EMD were performed. The affinity constant is 11.1 nM.

• Featured Product
• KD/KO Validated

Emerin Uni-rAb^® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 82888-3-PBS

Clone No.230089B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

EMD, 230089B12, EDMD, LEMD5, STA

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Product Information

82888-3-PBS targets Emerin in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0236

  Product name: Recombinant human EMD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 66-252 aa of BC000738

  Sequence: STRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGN

• Full Name: emerin
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC000738
• Gene Symbol: EMD
• Gene ID (NCBI): 2010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P50402
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.