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Emerin Recombinant monoclonal antibody

Emerin Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 82888-3-RR
Clone No.230089B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

EMD, 230089B12, EDMD, LEMD5, STA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 750
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82888-3-RR targets Emerin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0236

Product name: Recombinant human EMD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 66-252 aa of BC000738

Sequence: STRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGN

Predict reactive species
Full Name emerin
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC000738
Gene Symbol EMD
Gene ID (NCBI) 2010
RRIDAB_3670610
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP50402
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Emerin antibody 82888-3-RRDownload protocol
IHC protocol for Emerin antibody 82888-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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