EMG1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EMG1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11965-1-AP

Synonyms

NEP1, Grcc2f, EC:2.1.1.-, C2F, 18S rRNA Psi1248 methyltransferase

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Validation Data Gallery

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  WB analysis of HEK-293 using 11965-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:3000 incubated at 4 degree celsius over night.

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  WB analysis of HEK-293 using 11965-1-AP

  WB result of EMG1 antibody (11965-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMG1 transfected HEK-293 cells.

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  WB analysis of HeLa using 11965-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11965-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11965-1-AP

  WB result of EMG1 antibody (11965-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMG1 transfected HeLa cells.

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  IP experiment of HeLa using 11965-1-AP

  IP result of anti-EMG1 (IP:11965-1-AP, 4ug; Detection:11965-1-AP 1:400) with HeLa cells lysate 1080ug.

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  IF Staining of HeLa using 11965-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EMG1 antibody (11965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

11965-1-AP targets EMG1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EMG1 fusion protein Ag2575
• Full Name: EMG1 nucleolar protein homolog (S. cerevisiae)
• Calculated Molecular Weight: 244 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC055314
• Gene Symbol: EMG1
• Gene ID (NCBI): 10436
• RRID: AB_874093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EMG1, also named as NEP1, is a highly conserved protein initially identified as "Essential for Mitotic Growth" in yeast. EMG1 is required for maturation of the 18S rRNA and biogenesis of the 40S ribosomal subunit. EMG1 is the methyltransferase in the biosynthesis of m1acp3-Psi in eukaryotic 18S rRNAs. It has an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity. A mutation in human EMG1 gene causes Bowen-Conradi syndrome (BCS), an autosomal recessive disorder characterized by severely impaired prenatal and postnatal growth, profound psychomotor retardation, and death in early childhood.

Publications

Species	Application	Title
		Mol Cell Proteomics; Nucleolar enrichment of brain proteins with critical roles in human neurodevelopment.; Authors - Lukasz Slomnicki
mouse	IF	Biochim Biophys Acta; Growth arrest in the ribosomopathy, Bowen-Conradi syndrome, is due to dramatically reduced cell proliferation and a defect in mitotic progression.; Authors - Joy Armistead
goat	WB	Int J Mol Sci; Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods; Authors - Zhipeng Sun