EMILIN3 Polyclonal antibody

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  IP experiment of COLO 320 using 21308-1-AP

  IP result of anti-EMILIN3 (IP:21308-1-AP, 4ug; Detection:21308-1-AP 1:600) with COLO 320 cells lysate 1920 ug.

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  IHC staining of mouse testis using 21308-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21308-1-AP (EMILIN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 21308-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21308-1-AP (EMILIN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EMILIN3 Polyclonal Antibody for IHC, IP, ELISA

Cat No. 21308-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, ELISA

Synonyms

C20orf130, EMILIN 3, EMILIN 5, EMILIN5

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Tested Applications

• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21308-1-AP targets EMILIN3 in IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15925

  Product name: Recombinant human EMILIN3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 418-766 aa of BC101045

  Sequence: DELTRLSAAMLEGGVDGLLEGLETLNGTEGGARGCCLRLDMGGWGVGGFGTMLEERVQSLEERLATLAGELSHDSASPGRSARPLVQTELAVLEQRLVSLETSCTPSTTSAILDSLVAEVKAWQSRSEALLRQVASHAALLQQLNGTVAEVQGQLAEGTGSSLQGEITLLKVNLNSVSKSLTGLSDSVSQYSDAFLAANTSLDERERKVEAEVQAIQEQVSSQGSRLQAGHRQVLNLRGELEQLKAGVAKVASGLSRCQDTAQKLQHTVGHFDQRVAQVEGACRRLGLLAAGLDSLPTEPLRPREGLWSHVDQLNRTLAQHTQDIARLRDDLLDCQAQLAEQVRPGQAN

• Full Name: elastin microfibril interfacer 3
• Calculated Molecular Weight: 766 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC101045
• Gene Symbol: EMILIN3
• Gene ID (NCBI): 90187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NT22
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EMILIN3 (Elastin Microfibril Interfacer 3) is a protein-coding gene that encodes an extracellular matrix glycoprotein. It is a member of the EMILIN family and plays a role in the organization of the extracellular matrix, particularly in elastic fiber formation and extracellular matrix organization.