ENPEP Recombinant antibody

ENPEP Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85963-1-RR
Clone No.250264B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

AP A, APA, AP-A, CD249, EAP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue, rat lung tissue, rat kidney tissue, mouse lung tissue, =

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85963-1-RR targets ENPEP in applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ENPEP fusion protein Eg2736 Predict reactive species
Full Name glutamyl aminopeptidase (aminopeptidase A)
Calculated Molecular Weight109 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberNM_001977.3
Gene Symbol ENPEP
Gene ID (NCBI) 2028
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07075
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.

Protocols

Product Specific Protocols
WB protocol for ENPEP antibody 85963-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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