ENPEP Recombinant antibody

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  WB analysis using 85963-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 85963-1-RR

  rat lung tissue were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 85963-1-RR

  human kidney tissue were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ENPEP Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85963-1-RR

Clone No.250264B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

AP A, APA, AP-A, CD249, EAP

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human kidney tissue, rat lung tissue, rat kidney tissue, mouse lung tissue, =

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85963-1-RR targets ENPEP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ENPEP fusion protein Eg2736
• Full Name: glutamyl aminopeptidase (aminopeptidase A)
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_001977.3
• Gene Symbol: ENPEP
• Gene ID (NCBI): 2028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07075
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.