Tested Applications
Positive WB detected in | mouse kidney tissue, human kidney tissue, rat lung tissue, rat kidney tissue, mouse lung tissue, = |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85963-1-RR targets ENPEP in applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | ENPEP fusion protein Eg2736 Predict reactive species |
Full Name | glutamyl aminopeptidase (aminopeptidase A) |
Calculated Molecular Weight | 109 kDa |
Observed Molecular Weight | 140 kDa |
GenBank Accession Number | NM_001977.3 |
Gene Symbol | ENPEP |
Gene ID (NCBI) | 2028 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q07075 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.
Protocols
Product Specific Protocols | |
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WB protocol for ENPEP antibody 85963-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |