Anti-Mouse ENPEP Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of mouse bone marrow cells using 98489-3-RR (same clone as 98489-3-PBS)

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse ENPEP Rabbit RecAb (98489-3-RR, Clone:250659C1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Mouse CD45R. Cells were not fixed. This data was developed using the same antibody clone with 98489-3-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse bone marrow cells using 98489-3-RR (same clone as 98489-3-PBS)

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse ENPEP Rabbit RecAb (98489-3-RR, Clone: 250659C1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Mouse CD45R. Cells were not fixed. This data was developed using the same antibody clone with 98489-3-PBS in a different storage buffer formulation.

ENPEP Uni-rAb^® Recombinant Antibody for FC

Cat No. 98489-3-PBS

Clone No.250659C1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Aminopeptidase A, AP-A, BP-1/6C3 antigen, CD249, EAP

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Product Information

98489-3-PBS targets ENPEP in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4233

  Product name: Recombinant Mouse ENPEP protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 41-945 aa of NM_007934.3

  Sequence: RSCEQDTTPAPSQPPPEASTALPPQDQNVCPDSEDESGEWKNFRLPDFINPVHYDLEVKALMEEDRYTGIVTISVNLSKPTRDLWLHIRETKITKLPELRRPSGEQVPIRRCFEYKKQEYVVIQAAEDLAATSGDSVYRLTMEFKGWLNGSLVGFYKTTYMEDGQIRSIAATDHEPTDARKSFPCFDEPNKKSTYSISIIHPKEYSALSNMPEEKSEMVDDNWKKTTFVKSVPMSTYLVCFAVHRFTAIERKSRSGKPLKVYVQPNQKETAEYAANITQAVFDYFEDYFAMEYALPKLDKIAIPDFGTGAMENWGLVTYRETNLLYDPLLSASSNQQRVASVVAHELVHQWFGNTVTMDWWDDLWLNEGFASFFEFLGVNHAEKDWQMLSQVLLEDVFPVQEDDSLMSSHPVVVTVSTPAEITSVFDGISYSKGASILRMLQDWITPEKFQKGCQIYLKKFQFANAKTSDFWDSLQEASNLPVKEVMDTWTSQMGYPVVTVSGRQNITQKRFLLDSKADPSQPPSELGYTWNIPVRWADNDNSRITVYNRLDKGGITLNANLSGDAFLKINPDHIGFYRVNYEGGTWDWIAEALSSNHTRFSAADRSSFIDDAFALARAQLLNYKIALNLTMYLKSEEDFLPWERVISSVSYIISMFEDDRELYPMIETYFQGQVKPVADLLGWQDTGSHITKLLRASILGFACKMGDREALGNASQLFDSWLKGSASIPVNLRLLVYRYGMQNSGNEAAWNYTLEQYQKTSLAQEKEKLLYGLASVKDVKLLARYLEMLKDPNIIKTQDVFTVIRYISYNSYGKTMAWNWIQLNWDYLVSRFTINDRYLGRIVTIAEPFNTELQLWQMQSFFAKYPNAGAGAKPREQVLETVKNNIEWLNVNRQSIREWFASLP

• Full Name: glutamyl aminopeptidase
• Calculated Molecular Weight: 108 kDa
• GenBank Accession Number: NM_007934.3
• Gene Symbol: Enpep
• Gene ID (NCBI): 13809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16406
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.