CD39/ENTPD1 Polyclonal antibody

CD39/ENTPD1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14211-1-AP

Synonyms

ENTPD1, Ecto apyrase, EC:3.6.1.5, CD39, ATP-DPH

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Validation Data Gallery

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  WB analysis of mouse placenta using 14211-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 14211-1-AP (ENTPD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14211-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14211-1-AP

  human placenta lysates were subjected to SDS PAGE followed by western blot with 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human placenta using 14211-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD39/ENTPD1 antibody (14211-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14211-1-AP

  Immunofluorescent analysis of HeLa cells using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14211-1-AP

  Immunofluorescent analysis of HeLa cells using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse placenta tissue, human placenta tissue
• Positive IHC detected in: human placenta tissue, human tonsillitis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

14211-1-AP targets CD39/ENTPD1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD39/ENTPD1 fusion protein Ag5429
• Full Name: ectonucleoside triphosphate diphosphohydrolase 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 50 kDa，70-100 kDa
• GenBank Accession Number: BC047664
• Gene Symbol: CD39
• Gene ID (NCBI): 953
• RRID: AB_10638609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing.

Publications

Species	Application	Title
human,mouse	WB,IF	Int J Biol Sci; CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence; Authors - Zhenni Liu
human	IF	Genomics Proteomics Bioinformatics; Single-cell Immune Landscape of Human Recurrent Miscarriage.; Authors - Feiyang Wang
human	IHC	Oncotarget; Expression of CD38 in myeloma bone niche: A rational basis for the use of anti-CD38 immunotherapy to inhibit osteoclast formation.; Authors - Federica Costa
rat	IHC	Am J Physiol Gastrointest Liver Physiol; Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity.; Authors - Moyuru Hayashi
human	WB	BMC Med Genomics; Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis.; Authors - Xing-Cheng Zhao
rat	WB	J Gerontol A Biol Sci Med Sci; Hypoxanthine Induces Signs of Bladder Aging with Voiding dysfunction and Lower Urinary Tract Remodeling; Authors - Lori A Birder