ENTPD8 Polyclonal antibody

• 

  WB analysis using 21010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 21010-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ENTPD8 Polyclonal Antibody for WB, ELISA

Cat No. 21010-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, ELISA

Synonyms

E NTPDase 8, ENTPD8, GLSR2492, NTPDase 8, NTPDase8, UNQ2492

---

Tested Applications

• Positive WB detected in: 37°C incubated HepG2 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21010-1-AP targets ENTPD8 in WB, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15224

  Product name: Recombinant human ENTPD8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 131-350 aa of BC141810

  Sequence: LSRKNSSQARDIFAAVTQVLGRSPVDFWGAELLAGQAEGAFGWITVNYGLGTLVKYSFTGEWIQPPEEMLVGALDMGGASTQITFVPGGPILDKSTQADFRLYGSDYSVYTHSYLCFGRDQMLSRLLVGLVQSRPAALLRHPCYLSGYQTTLALGPLYESPCVHATPPLSLPQNLTVEGTGNPGACVSAIRELFNFSSCQGQEDCAFDGVYQPPLRGQFY

• Full Name: ectonucleoside triphosphate diphosphohydrolase 8
• Calculated Molecular Weight: 495 aa, 54 kDa
• Observed Molecular Weight: 70 kDa, 54 kDa
• GenBank Accession Number: BC141810
• Gene Symbol: ENTPD8
• Gene ID (NCBI): 377841
• RRID: AB_10755288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5MY95
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD8 (Ectonucleoside triphosphate diphosphohydrolase 8) is also named as E-NTPDase 8, NTPDase8 and belongs to the GDA1/CD39 NTPase family. NTPDase8 is the canalicular ecto-ATPase/ATPDase and is responsible for the main hepatic NTP-Dase activity(PMID:17095758). The estimated molecular mass of expressed human ENTPD8 is 70-85 kDa which is N-glycosylated and after deglycosylation, it has a nearly identical molecular mass, 54 kDa(PMID:16752921;7781224). It has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 49 kDa. This antibody is specific to ENTPD8.