EPB41L2 Polyclonal antibody

EPB41L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15437-1-AP

Synonyms

Erythrocyte membrane protein band 4.1-like 2, EPB41 L2, Band 4.1-like protein 2, Band 4.1 like protein 2, 4.1G

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Validation Data Gallery

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  WB analysis of HeLa using 15437-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15437-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15437-1-AP

  IP result of anti-EPB41L2 (IP:15437-1-AP, 4ug; Detection:15437-1-AP 1:4000) with HEK-293 cells lysate 1610 ug.

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  IHC staining of human ovary tumor using 15437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15437-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EPB41L2 antibody (15437-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 15437-1-AP

  Immunofluorescent analysis of HeLa cells using 15437-1-AP (EPB41L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15437-1-AP targets EPB41L2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPB41L2 fusion protein Ag3647
• Full Name: erythrocyte membrane protein band 4.1-like 2
• Calculated Molecular Weight: 112 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC034718
• Gene Symbol: EPB41L2
• Gene ID (NCBI): 2037
• RRID: AB_10951110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPB41L2, also known as 4.1G, is a member of the protein 4.1 family, which generally serves as adaptors linking transmembrane proteins to the cytoskeleton(PMID: 21482674).