EPB41L2 Polyclonal antibody

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• EPB41L2 Polyclonal antibody (15437-1-AP)

EPB41L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15437-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

4.1 G, 4.1G, Band 4.1 like protein 2, Band 4.1-like protein 2, EPB41 L2

• 

  WB analysis of HeLa using 15437-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 15437-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 15437-1-AP

  IP result of anti-EPB41L2 (IP:15437-1-AP, 4ug; Detection:15437-1-AP 1:4000) with HEK-293 cells lysate 1610 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 15437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 15437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 15437-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EPB41L2 antibody (15437-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 15437-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EPB41L2 antibody (15437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15437-1-AP targets EPB41L2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3647

  Product name: Recombinant human EPB41L2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 334-633 aa of BC034718

  Sequence: ALLGSYTLQAELGDYDPEEHGSIDLSEFQFAPTQTKELEEKVAELHKTHRGLSPAQADSQFLENAKRLSMYGVDLHHAKDSEGVDIKLGVCANGLLIYKDRLRINRFAWPKILKISYKRSNFYIKVRPAELEQFESTIGFKLPNHRAAKRLWKVCVEHHTFYRLVSPEQPPKAKFLTLGSKFRYSGRTQAQTRQASTLIDRPAPHFERTSSKRVSRSLDGAPIGVMDQSLMKDFPGAAGEISAYGPGLVSIAVVQDGDGRREVRSPTKAPHLQLIEGKSSHETLNIVEEKSGQRLGKTKE

• Full Name: erythrocyte membrane protein band 4.1-like 2
• Calculated Molecular Weight: 112 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC034718
• Gene Symbol: EPB41L2
• Gene ID (NCBI): 2037
• RRID: AB_10951110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43491
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPB41L2, also known as 4.1G, is a member of the protein 4.1 family, which generally serves as adaptors linking transmembrane proteins to the cytoskeleton(PMID: 21482674).

Publications

What published studies show

The EPB41L2 Polyclonal antibody (Cat# 15437-1-AP) has 1 citation to date. It appears in Journal of Lipid Research (IF 4.9), published in 2026. The cited study investigates novel macrophage-specific biomarkers in MASH (metabolic dysfunction-associated steatohepatitis) using single-cell sequencing for diagnostic modeling. The antibody was applied in IHC and IF on human and mouse samples, placing its use within lipid metabolism and liver disease research.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human, mouse	IHC,IF	J Lipid Res; Unveiling novel macrophage-specific biomarkers in MASH through single-cell sequencing for diagnostic modeling.; Authors - Tingting Wang