EPB42 Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84921-3-RR (EPB42 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

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  IF Staining of HEL using 84921-3-RR (same clone as 84921-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEL cells using EPB42 antibody (84921-3-RR, Clone: 242412F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01708-1

  Cytometric bead array standard curve of MP01708-1, EPB42 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84921-2-PBS. Detection antibody: 84921-3-PBS. Standard: Ag23556. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP01708-2

  Cytometric bead array standard curve of MP01708-2, EPB42 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84921-1-PBS. Detection antibody: 84921-3-PBS. Standard: Ag23556. Range: 1.25-80 ng/mL.

EPB42 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 84921-3-PBS

Clone No.242412F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

242412F4, E42P, Erythrocyte protein 4.2, P4.2, PA

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Product Information

84921-3-PBS targets EPB42 as part of a matched antibody pair:

MP01708-1: 84921-2-PBS capture and 84921-3-PBS detection (validated in Cytometric bead array)

MP01708-2: 84921-1-PBS capture and 84921-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag23556

  Product name: Recombinant human EPB42 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 446-691 aa of BC096093

  Sequence: EVLERVEKEKMEREKDNGIRPPSLETASPLYLLLKAPSSLPLRGDAQISVTLVNHSEQEKAVQLAIGVQAVHYNGVLAAKLWRKKLHLTLSANLEKIITIGLFFSNFERNPPENTFLRLTAMATHSESNLSCFAQEDIAICRPHLAIKMPEKAEQYQPLTASVSLQNSLDAPMEDCVISILGRGLIHRERSYRFRSVWPENTMCAKFQFTPTHVGLQRLTVEVDCNMFQNLTNYKSVTVVAPELSA

• Full Name: erythrocyte membrane protein band 4.2
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC096093
• Gene Symbol: EPB42
• Gene ID (NCBI): 2038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16452
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EPB42, also known as Protein 4.2, is amongst the most abundant protein components of the erythrocyte membrane, representing approximately 5% membrane content or ~2.5 × 105 copies/cell. EPB42 binds to the cytoplasmic domain of band 3, which serves as a site for the anchorage of several membrane skeletal proteins(PMID: 19269200, 12049649). EPB42 is a 72 kDa peripheral membrane protein.