EPLIN Monoclonal antibody, PBS Only

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  WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

  WB result of EPLIN antibody (66071-1-Ig, 1:2000) with si-Control and si-EPLIN transfected HEK293 cells. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66071-1-Ig (same clone as 66071-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66071-1-Ig (same clone as 66071-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

  Immunofluorescent analysis of Hela cells using 66071-1-Ig (EPLIN antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

EPLIN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66071-1-PBS

Clone No.6E6E6

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

LIMA1, 6E6E6, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624

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Product Information

66071-1-PBS targets EPLIN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13288

  Product name: Recombinant human LIMA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 261-401 aa of BC001247

  Sequence: KPLSPDSRASSLSESSPPKAMKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAK

• Full Name: LIM domain and actin binding 1
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90 kDa, 110 kDa
• GenBank Accession Number: BC001247
• Gene Symbol: EPLIN
• Gene ID (NCBI): 51474
• RRID: AB_11043341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UHB6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.