Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, COLO 320 cells, HEK-293 cells, human brain tissue, HaCaT cells, SCaBER cells, MDA-MB-231 cells, SK-BR-3 cells, HT-29 cells, pig colon tissue, rat colon tissue, HSC-T6 cells, ROS1728 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66071-1-Ig targets EPLIN in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13288 Product name: Recombinant human LIMA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 261-401 aa of BC001247 Sequence: KPLSPDSRASSLSESSPPKAMKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAK Predict reactive species |
| Full Name | LIM domain and actin binding 1 |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | 90 kDa, 110 kDa |
| GenBank Accession Number | BC001247 |
| Gene Symbol | EPLIN |
| Gene ID (NCBI) | 51474 |
| RRID | AB_11043341 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UHB6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EPLIN antibody 66071-1-Ig | Download protocol |
| IHC protocol for EPLIN antibody 66071-1-Ig | Download protocol |
| WB protocol for EPLIN antibody 66071-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LIMA1 antibody (Cat# 66071-1-Ig) has 4 total citations. These appear in Advanced Science, Cell Molecular Biology Letters, Cell Biology and Toxicology, and Frontiers in Cellular and Infection Microbiology. Research fields span hepatic lipid metabolism and steatotic liver disease, hepatocyte-derived extracellular vesicles in liver fibrosis, hepatocellular carcinoma progression via Wnt-β-catenin/Hippo signaling, and parasitic infection (Echinococcus granulosus). Applications include WB, IHC, IF, and CoIP across human and mouse samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease | ||
Cell Mol Biol Lett Lipotoxic hepatocyte derived LIMA1 enriched small extracellular vesicles promote hepatic stellate cells activation via inhibiting mitophagy
| ||
Cell Biol Toxicol M6A modification-mediated LIMA1 promotes the progression of hepatocellular carcinoma through the wnt-βcatenin/Hippo pathway | ||
Front Cell Infect Microbiol Screening and identification of protein 29 of Echinococcus granulosus interacting molecules |
