CoraLite® Plus 488-conjugated EPLIN Monoclonal antibody

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  IF Staining of HeLa using CL488-66071

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66071 (EPLIN antibody) at dilution of 1:100.

• Featured Product
• KD/KO Validated

EPLIN Monoclonal Antibody for IF/ICC

Cat No. CL488-66071

Clone No.6E6E6

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC

Synonyms

EPLIN, FLJ38853, LIM domain and actin binding 1, LIMA1, SREBP3

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66071 targets EPLIN in IF/ICC applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13288

  Product name: Recombinant human LIMA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 261-401 aa of BC001247

  Sequence: KPLSPDSRASSLSESSPPKAMKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAK

• Full Name: LIM domain and actin binding 1
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90 kDa, 110 kDa
• GenBank Accession Number: BC001247
• Gene Symbol: EPLIN
• Gene ID (NCBI): 51474
• RRID: AB_2883250
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UHB6
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.