EPS8 Recombinant antibody, PBS Only

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  WB analysis using 85184-5-RR (same clone as 85184-5-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85184-5-RR (EPS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85184-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85184-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85184-5-RR against Human EPS8 were performed. The affinity constant is 27.9 nM.

EPS8 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85184-5-PBS

Clone No.242835G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Epidermal growth factor receptor kinase substrate 8

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Product Information

85184-5-PBS targets EPS8 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EPS8 fusion protein Ag3121
• Full Name: epidermal growth factor receptor pathway substrate 8
• Calculated Molecular Weight: 822 aa, 92 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC030010
• Gene Symbol: EPS8
• Gene ID (NCBI): 2059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q12929
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Epidermal growth factor receptor Pathway Substrate 8 (EPS8) is a crucial regulator of the actin cytoskeleton dynamics accompanying cell motility and invasion. This protein contains one PH domain and one SH3 domain leading to its binding activity with multiple cellular targets. EPS8 can function as a unique actin capping protein specifically required for dendritic cell migration and plays roles in the regulation of axonal filopodia in neuronal development and synapse formation. The EPS8 gene may contribute to the development of a subset of colorectal cancers and could have applications in diagnosis and treatment.