EPS8L1 Polyclonal antibody

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• EPS8L1 Polyclonal antibody (33149-1-AP)

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  WB analysis using 33149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33149-1-AP (EPS8L1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

EPS8L1 Polyclonal Antibody for WB, ELISA

Cat No. 33149-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DRC3, Epidermal growth factor receptor kinase substrate 8-like protein 1, Epidermal growth factor receptor pathway substrate 8-related protein 1, EPS8-like protein 1, EPS8R1

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Tested Applications

• Positive WB detected in: HCT 116 cells, HT-29 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33149-1-AP targets EPS8L1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38144

  Product name: Recombinant human EPS8L1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 247-596 aa of BC015763

  Sequence: LTSDAVALLRDNVTPRENELWTSLGDSWTRPGLELSPEEGPPYRPEFFSGWEPPVTDPQSRAWEDPVEKQLQHERRRRQQSAPQVAVNGHRDLEPESEPQLESETAGKWVLCNYDFQARNSSELSVKQRDVLEVLDDSRKWWKVRDPAGQEGYVPYNILTPYPGPRLHHSQSPARSLNSTPPPPPAPAPAPPPALARPRWDRPRWDSCDSLNGLDPSEKEKFSQMLIVNEELQARLAQGRSGPSRAVPGPRAPEPQLSPGSDASEVRAWLQAKGFSSGTVDALGVLTGAQLFSLQKEELRAVSPEEGARVYSQVTVQRSLLEDKEKVSELEAVMEKQKKKVEGEVEMEVI

• Full Name: EPS8-like 1
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC015763
• Gene Symbol: EPS8L1
• Gene ID (NCBI): 54869
• RRID: AB_3742872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8TE68
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPS8L1 encodes Epidermal Growth Factor Receptor Kinase Substrate 8-like Protein 1, a protein related to EPS8 and positioned within the EPS8 signaling adaptor family. Its biology is centered on signaling at the cell cortex, where it is connected to ruffle membrane and cytosolic compartments and is associated with positive regulation of ruffle assembly. The protein contains multiple interaction-related domains, including a PTB/PI domain, phosphotyrosine-binding domain, SH3 domain, and SAM domain, supporting a role in signal-dependent molecular interactions.