EPSTI1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EPSTI1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11627-1-AP

Synonyms

Epithelial-stromal interaction protein 1

---

Validation Data Gallery

• 

  WB analysis of mouse spleen using 11627-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11627-1-AP (EPSTI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 11627-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 11627-1-AP (EPSTI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: mouse small intestine tissue, mouse spleen tissue
• Positive IHC detected in: human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

11627-1-AP targets EPSTI1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPSTI1 fusion protein Ag2157
• Full Name: epithelial stromal interaction 1 (breast)
• Calculated Molecular Weight: 410 aa, 47 kDa
• Observed Molecular Weight: 38-47 kDa
• GenBank Accession Number: BC023660
• Gene Symbol: EPSTI1
• Gene ID (NCBI): 94240
• RRID: AB_2877786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Real-time PCR revealed the highest expression in the placenta; strong expression in the small intestine, spleen, salivary gland, and testes; and low expression levels in normal breast and several other tissues. The expression of EPSTI1 was upregulated (range 5.6-72.1) in all 14 breast carcinomas tested. Microdissection revealed that both tumor and stromal tissue overexpressed EPSTI1. (PubMed: 11991720)

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Acta Pharmacol Sin; EPSTI1 promotes monocyte adhesion to endothelial cells in vitro via upregulating VCAM-1 and ICAM-1 expression.; Authors - Yan-Rou Bei ; KD Validated
human	WB	Elife; KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition.; Authors - Chaitra Rao
human	IHC	Immunobiology; Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations; Authors - Tiantian Cai
human	WB	Mol Cancer; Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study.; Authors - Erfei Chen
human	IHC	Oxid Med Cell Longev; Systematic Analysis of Molecular Subtypes Based on the Expression Profile of Immune-Related Genes in Pancreatic Cancer; Authors - Mujing Ke