Tested Applications
| Positive WB detected in | K-562 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13821-1-AP targets ARTS1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4918 Product name: Recombinant human ERAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 642-941 aa of BC030775 Sequence: NAFQLVSIGKLSIEKALDLSLYLKHETEIMPVFQGLNELIPMYKLMEKRDMNEVETQFKAFLIRLLRDLIDKQTWTDEGSVSERMLRSQLLLLACVHNYQPCVQRAEGYFRKWKESNGNLSLPVDVTLAVFAVGAQSTEGWDFLYSKYQFSLSSTEKSQIEFALCRTQNKEKLQWLLDESFKGDKIKTQEFPQILTLIGRNPVGYPLAWQFLRKNWNKLVQKFELGSSSIAHMVMGTTNQFSTRTRLEEVKGFFSSLKENGSQLRCVQQTIETIEENIGWMDKNFDKIRVWLQSEKLERM Predict reactive species |
| Full Name | endoplasmic reticulum aminopeptidase 1 |
| Calculated Molecular Weight | 107 kDa |
| Observed Molecular Weight | 106 kDa |
| GenBank Accession Number | BC030775 |
| Gene Symbol | ARTS1/ERAP1 |
| Gene ID (NCBI) | 51752 |
| RRID | AB_2293531 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZ08 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARTS1 is an endoplasmic reticulum-associated aminopeptidase (also known as ERAP1). ERAP1 is induced by interferon-gama and can degrade peptides 9-15 residues long(PMID:18987748).ARTS1 is a 100 kDa, monomeric, soluble zinc aminopeptidase. It can be glycosylated to 120KDa and form homodimers and heterodimers(PMID:15908954).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARTS1 antibody 13821-1-AP | Download protocol |
| IP protocol for ARTS1 antibody 13821-1-AP | Download protocol |
| WB protocol for ARTS1 antibody 13821-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ERAP1 antibody (Cat# 13821-1-AP) has 3 total citations published in Cell Communications & Signaling, Autoimmunity, and Journal of Immunology. These citations span research fields including antigen presentation, autoimmunity (ankylosing spondylitis), peptide trimming, and mucosal immunity in zebrafish models. Applications cited include Western blotting in human and zebrafish species.
| Species | Application | Title |
|---|---|---|
Cell Commun Signal p53 regulates early mucosal immunity via antigen presentation in zebrafish intestine post-SVCV vaccination. | ||
Autoimmunity Endoplasmic reticulum aminopeptidase-1 alleles associated with increased risk of ankylosing spondylitis reduce HLA-B27 mediated presentation of multiple antigens. | ||
