ARTS1 Polyclonal antibody

ARTS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13821-1-AP

Synonyms

A LAP, ALAP, Aminopeptidase PILS, APPILS, ARTS 1, ARTS1, ERAAP, ERAAP1, ERAP1, KIAA0525, PILS AP, PILSAP, Puromycin insensitive leucyl specific aminopeptidase

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Validation Data Gallery

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  WB analysis of K-562 using 13821-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13821-1-AP (ARTS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13821-1-AP

  IP result of anti-ARTS1 (IP:13821-1-AP, 4ug; Detection:13821-1-AP 1:600) with Jurkat cells lysate 3600ug.

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  IHC staining of human ovary tumor using 13821-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13821-1-AP (ARTS1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 13821-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13821-1-AP (ARTS1 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13821-1-AP targets ARTS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARTS1 fusion protein Ag4918
• Full Name: endoplasmic reticulum aminopeptidase 1
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 106 kDa
• GenBank Accession Number: BC030775
• Gene Symbol: ARTS1/ERAP1
• Gene ID (NCBI): 51752
• RRID: AB_2293531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARTS1 is an endoplasmic reticulum-associated aminopeptidase (also known as ERAP1). ERAP1 is induced by interferon-gama and can degrade peptides 9-15 residues long(PMID:18987748).ARTS1 is a 100 kDa, monomeric, soluble zinc aminopeptidase. It can be glycosylated to 120KDa and form homodimers and heterodimers(PMID:15908954).

Publications

Species	Application	Title
human	WB	J Immunol; ERAP1-ERAP2 Dimerization Increases Peptide-Trimming Efficiency.; Authors - Irini Evnouchidou
		Autoimmunity; Endoplasmic reticulum aminopeptidase-1 alleles associated with increased risk of ankylosing spondylitis reduce HLA-B27 mediated presentation of multiple antigens.; Authors - Seregin Sergey S SS