HER2/ErbB2 Polyclonal antibody
HER2/ErbB2 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
WB, IHC, IF,ELISA
Cat no : 51105-1-AP
|Positive WB detected in||HeLa cells, MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, Breast Cancer slides |
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
51105-1-AP targets HER2/ErbB2 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Full Name||v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)|
|Calculated molecular weight||185 kDa|
|Observed molecular weight||185 kDa|
|GenBank accession number||BC167147|
|Gene ID (NCBI)||2064|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the EGF receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (11-26-2018)
We found two bands between 250 and 150kDa and they are very close to each other. The lower band is the right band but the topper band is too dominant in different breast cancer cells we detected.