HER2/ErbB2 Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01080-1

  Sandwich ELISA standard curve of MP01080-1, Human HER2/ErbB2 Recombinant Matched Antibody Pair - PBS only. 84182-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02350. 84182-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

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  Sample test of MP01080-1

  Serum of six individual healthy human donors was measured. The human HER2/ErbB2 concentration of detected samples was determined to be 8,952.9 pg/mL with a range of 4,726.1 - 15,157.7 pg/mL.

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  Sample test of MP01080-1

  Human milk of six individual healthy human donors was measured. The human HER2/ErbB2 concentration of detected samples was determined to be 969.9 pg/mL with a range of 654.3 - 1,208.0 pg/mL.

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  Sample test of MP01080-1

  The mean HER2/ErbB2 concentration was determined to be 6,844.8 pg/mL in human placenta tissue extract based on a 2.9 mg/mL extract load.

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  Cytometric bead array standard curve of MP01080-1

  Cytometric bead array standard curve of MP01080-1, HER2/ERBB2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84182-2-PBS. Detection antibody: 84182-3-PBS. Standard: RP02350. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84182-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 84182-3-PBS against Human HER2/ErbB2 was performed. The affinity constant is 0.813 nM.

HER2/ErbB2 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84182-3-PBS

Clone No.241409H4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

ERBB2, CD340, HER 2, HER 2/neu, HER2

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Product Information

84182-3-PBS targets HER2/ErbB2 as part of a matched antibody pair:

MP01080-1: 84182-2-PBS capture and 84182-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Calculated Molecular Weight: 1255 aa, 138 kDa
• GenBank Accession Number: BC156755
• Gene Symbol: HER2/ErbB2
• Gene ID (NCBI): 2064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04626
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.