Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22211-1-AP targets ERC1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17536 Product name: Recombinant human ERC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 985-1116 aa of BC132782 Sequence: DNYEDDHFKSSHSNQTNHKPSPDQIIQPLLELDQNRSKLKLYIGHLTTLCHDRDPLILRGLTPPASYNLDDDQAAWENELQKMTRGQLQDELEKGERDNAELQEFANAILQQIADHCPDILEQVVNALEESS Predict reactive species |
| Full Name | ELKS/RAB6-interacting/CAST family member 1 |
| Calculated Molecular Weight | 1116 aa, 128 kDa |
| Observed Molecular Weight | 124-135 kDa |
| GenBank Accession Number | BC132782 |
| Gene Symbol | ERC1 |
| Gene ID (NCBI) | 23085 |
| RRID | AB_11232409 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IUD2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERC is an acronym based on previous separate namings of members of this protein family, ELKS, Rab6IP2 and CAST (PMID: 12391317). They are known to bind RIMs, the active zone proteins that regulate neurotransmitter release. ERC1 (ELKS) is an essential regulatory subunit of the IKK complex and likely functions by recruiting IκBα to the complex (PMID: 15218148). Multiple isoforms of ERC1 mRNA are generated by alternative splicing (PMID: 12203787). There are two known splice variants of ERC1 protein that differ at their C termini, ERC1a and ERC1b. ERC1a is ubiquitously expressed, whereas ERC1b is brain-specific (PMID: 12391317; 12923177).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ERC1 antibody 22211-1-AP | Download protocol |
| IHC protocol for ERC1 antibody 22211-1-AP | Download protocol |
| WB protocol for ERC1 antibody 22211-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ERC1 Polyclonal antibody (Cat# 22211-1-AP) has 7 citations published in Nature Communications, Translational Psychiatry, Journal of Cell Biology (×2), Scientific Reports, Journal of Biological Chemistry, and Journal of Cell Science. Research fields span microtubule dynamics and protein condensates, neuropsychiatry (G6PD deficiency/schizophrenia), intracellular trafficking and secretion, mechanical force signaling at the Golgi, mass spectrometry-based protein binding studies, cytokinesis regulation, and insulin secretion in pancreatic β-cells.
| Species | Application | Title |
|---|---|---|
Nat Commun CLASP-mediated competitive binding in protein condensates directs microtubule growth | ||
Transl Psychiatry G6PD deficiency in brain induces schizophrenia-like behaviors and synaptic dysfunction. | ||
Sci Rep Facilitating identification of minimal protein binding domains by cross-linking mass spectrometry. | ||
J Biol Chem MICAL3 flavoprotein monooxygenase forms a complex with centralspindlin and regulates cytokinesis.
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Reviews
What customers say
One reviewer (Sammy El-Mansi) gave this antibody a 5-star rating after using it for immunofluorescence on HUVEC cells at a 1:200 dilution. The reviewer reported that the antibody performed well, confirming its reliability for IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sammy (Verified Customer) (11-20-2023) | Ab performs well by Immunofluorescence.
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