ERCC1 Monoclonal antibody

ERCC1 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1H4D5

Cat no : 66275-1-Ig

Synonyms

COFS4, ERCC1, UV20



Tested Applications

Positive WB detected inA431 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66275-1-Ig targets ERCC1 in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERCC1 fusion protein Ag6417
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Calculated molecular weight 33 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC052813
Gene symbol ERCC1
Gene ID (NCBI) 2067
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005)

Protocols

Product Specific Protocols
WB protocol for ERCC1 antibody 66275-1-IgDownload protocol
IF protocol for ERCC1 antibody 66275-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

PMID: 31541631

Authors - Bin Zhu