ERCC6/CSB Polyclonal antibody

• Featured Product
• KD/KO Validated

ERCC6/CSB Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24291-1-AP

Synonyms

ARMD5, ATP dependent helicase ERCC6, CKN2, Cockayne syndrome protein CSB, COFS, COFS1, CSB, ERCC6, ERCC6/CSB, RAD26

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Validation Data Gallery

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  WB analysis of HeLa using 24291-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24291-1-AP (ERCC6/CSB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24291-1-AP (ERCC6/CSB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24291-1-AP

  IP result of anti-ERCC6/CSB (IP:24291-1-AP, 4ug; Detection:24291-1-AP 1:300) with HeLa cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, PC-3 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

24291-1-AP targets ERCC6/CSB in WB, IP, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERCC6/CSB fusion protein Ag18009
• Full Name: excision repair cross-complementing rodent repair deficiency, complementation group 6
• Calculated Molecular Weight: 1493 aa, 168 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC127104
• Gene Symbol: ERCC6/CSB
• Gene ID (NCBI): 2074
• RRID: AB_2879476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; A Gene Expression Signature to Predict Nucleotide Excision Repair Defects and Novel Therapeutic Approaches.; Authors - Rongbin Wei
mouse	WB,IF	Front Mol Biosci; Blockage of ERCC6 Alleviates Spinal Cord Injury Through Weakening Apoptosis, Inflammation, Senescence, and Oxidative Stress.; Authors - Peng Zou ; KD Validated
human	WB	Life Sci Alliance; Elevated glucose increases genomic instability by inhibiting nucleotide excision repair.; Authors - Alexandra K Ciminera
human	WB,IP	Nucleic Acids Res; Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons; Authors - Hao Li