ERCC6/CSB Polyclonal antibody

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• ERCC6/CSB Polyclonal antibody (24291-1-AP)

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• KD/KO Validated

ERCC6/CSB Polyclonal Antibody for WB, IP, ELISA

Cat No. 24291-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IP, ELISA

Synonyms

ERCC6, ARMD5, ATP dependent helicase ERCC6, Chimeric CSB-PGBD3 protein, Chimeric ERCC6-PGBD3 protein

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  WB analysis of HeLa using 24291-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24291-1-AP (ERCC6/CSB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 24291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24291-1-AP (ERCC6/CSB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 24291-1-AP

  WB result of ERCC6/CSB antibody (24291-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERCC6/CSB transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 24291-1-AP

  IP result of anti-ERCC6/CSB (IP:24291-1-AP, 4ug; Detection:24291-1-AP 1:300) with HeLa cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, PC-3 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24291-1-AP targets ERCC6/CSB in WB, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18009

  Product name: Recombinant human ERCC6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1144-1493 aa of BC127104

  Sequence: DESIDEKLGLSYKRERPSQAQTEAFWENKQMENNFYKHKSKTKHHSVAEEETLEKHLRPKQKPKNSKHCRDAKFEGTRIPHLVKKRRYQKQDSENKSEAKEQSNDDYVLEKLFKKSVGVHSVMKHDAIMDGASPDYVLVEAEANRVAQDAPKALRLSRQRCLGAVSGVPTWTGHRGISGAPAGKKSRFGKKRNSNFSVQHPSSTSPTEKCQDGIMKKEGKDNVPEHFSGRAEDADSSSGPLASSSLLAKMRARNHLILPERLESESGHLQEASALLPTTEHDDLLVEMRNFIAFQAHTDGQASTREILQEFESKLSASQSCVFRELLRNLCTFHRTSGGEGIWKLKPEYC

• Full Name: excision repair cross-complementing rodent repair deficiency, complementation group 6
• Calculated Molecular Weight: 1493 aa, 168 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC127104
• Gene Symbol: ERCC6/CSB
• Gene ID (NCBI): 2074
• RRID: AB_2879476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q03468
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The ERCC6/CSB Polyclonal antibody (Cat# 24291-1-AP) has 5 citations published in Nucleic Acids Research, International Journal of Molecular Sciences, Frontiers in Molecular Biosciences, Frontiers in Oncology, and Life Science Alliance. The research fields span alternative splicing regulation, nucleotide excision repair (NER) defects and therapeutic approaches, spinal cord injury recovery, prostate cancer-associated NER variants, and glucose-induced genomic instability. Applications cited include WB, IP, and IF.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Nucleic Acids Res; Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons; Authors - Hao Li
human	WB	Int J Mol Sci; A Gene Expression Signature to Predict Nucleotide Excision Repair Defects and Novel Therapeutic Approaches.; Authors - Rongbin Wei
mouse	WB,IF	Front Mol Biosci; Blockage of ERCC6 Alleviates Spinal Cord Injury Through Weakening Apoptosis, Inflammation, Senescence, and Oxidative Stress.; Authors - Peng Zou ; KD Validated
human	WB	Front Oncol; Studies on the functionality of the TC-NER ERCC6-M1097V protein variant frequently found in Louisiana patients with PCa upon UV damage.; Authors - Oluwatobi Ogundepo ; KD Validated
human	WB	Life Sci Alliance; Elevated glucose increases genomic instability by inhibiting nucleotide excision repair.; Authors - Alexandra K Ciminera