ERG Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• ERG Recombinant monoclonal antibody (88009-1-RR)

• 

  WB analysis using 88009-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88009-1-RR (ERG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MOLT-4 using 88009-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MOLT-4 cells using ERG antibody (88009-1-RR, Clone: 253522H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ERG Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88009-1-RR

Clone No.253522H8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

erg 3, Transcriptional regulator ERG, Transforming protein ERG

---

Tested Applications

• Positive WB detected in: VCaP cells, Jurkat cells, RAW 264.7 cells
• Positive IF/ICC detected in: MOLT-4 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88009-1-RR targets ERG in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5708

  Product name: Recombinant human ERG protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 141-479 aa of BC040168

  Sequence: QWLEWAVKEYGLPDVNILLFQNIDGKELCKMTKDDFQRLTPSYNADILLSHLHYLRETPLPHLTSDDVDKALQNSPRLMHARNTGGAAFIFPNTSVYPEATQRITTRPDLPYEPPRRSAWTGHGHPTPQSKAAQPSPSTVPKTEDQRPQLDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSSNSSCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPPESSLYKYPSDLPYMGSYHAHPQKMNFVAPHPPALPVTSSSFFAAPNPYWNSPTGGIYPNTRLPTSHMPSHLGTYY

• Full Name: v-ets erythroblastosis virus E26 oncogene homolog (avian)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC040168
• Gene Symbol: ERG
• Gene ID (NCBI): 2078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11308
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERG (ETS-related gene) is a member of the ETS family of transcription factors, which are downstream effectors of mitogenic signaling transduction pathway. ERG is a key regulator of cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. ERG is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia.