ERGIC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ERGIC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16108-1-AP

Synonyms

ERGIC 32, ERGIC1, ERGIC32, KIAA1181, NET24

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Validation Data Gallery

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  WB analysis of HepG2 using 16108-1-AP

  WB result of ERGIC1 antibody (16108-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC1 transfected HepG2 cells.

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  WB analysis of HepG2 using 16108-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16108-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 16108-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16108-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16108-1-AP

  IP result of anti-ERGIC1 (IP:16108-1-AP, 3ug; Detection:16108-1-AP 1:500) with HepG2 cells lysate 1720ug.

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  IHC staining of human liver cancer using 16108-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16108-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16108-1-AP

  Immunofluorescent analysis of HepG2 cells using 16108-1-AP (ERGIC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, L02 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

16108-1-AP targets ERGIC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERGIC1 fusion protein Ag9025
• Full Name: endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1
• Calculated Molecular Weight: 290 aa, 33 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC012766
• Gene Symbol: ERGIC1
• Gene ID (NCBI): 57222
• RRID: AB_2098435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of ERGIC1 remains largely unknown. ERGIC1 was highly expressed in most primary prostate tumors. It is an intriguing potential drug target especially for the ERG oncogene expressing tumors (PMID: 22761906). The MW of this protein is 32 kDa, and this antibody specially recognises the 32 kDa protein.

Publications

Species	Application	Title
human	WB	Cell Rep; Sequencing of captive target transcripts identifies the network of regulated genes and functions of primate-specific miR-522.; Authors - Shen Mynn Tan
human	WB	Cell Rep; Proteomic Profiling of Mammalian COPII and COPI Vesicles.; Authors - Frank Adolf
human	WB,IF	Nat Commun; Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2; Authors - Karsten Nalbach