ERGIC2 Polyclonal antibody, PBS Only

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• ERGIC2 Polyclonal antibody, PBS Only (11927-1-PBS)

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  WB analysis using 11927-1-AP (same clone as 11927-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11927-1-AP (ERGIC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11927-1-AP (same clone as 11927-1-PBS)

  WB result of ERGIC2 antibody (11927-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC2 transfected HeLa cells. This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 11927-1-AP (same clone as 11927-1-PBS)

  IP result of anti-ERGIC2 (IP:11927-1-AP, 4ug; Detection:11927-1-AP 1:1000) with HepG2 cells lysate 2900ug. This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11927-1-AP (same clone as 11927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11927-1-AP (same clone as 11927-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ERGIC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11927-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

11927-1-PBS targets ERGIC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2603

  Product name: Recombinant human ERGIC2 protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-377 aa of BC000887

  Sequence: MRRLNRKKTLSLVKELDAFPKVPESYVETSASGGTVSLIAFTTMALLTIMEFSVYQDTWMKYEYEVDKDFSSKLRINIDITVAMKCQYVGADVLDLAETMVASADGLVYEPTVFDLSPQQKEWQRMLQLIQSRLQEEHSLQDVIFKSAFKSTSTALPPREDDSSQSPNACRIHGHLYVNKVAGNFHITVGKAIPHPRGHAHLAALVNHESYNFSHRIDHLSFGELVPAIINPLDGTEKIAIDHNQMFQYFITVVPTKLHTYKISADTHQFSVTERERIINHAAGSHGVSGIFMKYDLSSLMVTVTEEHMPFWQFFVRLCGIVGGIFSTTGMLHGIGKFIVEIICCRFRLGSYKPVNSVPFEDGHTDNHLPLLENNTH

• Full Name: ERGIC and golgi 2
• Calculated Molecular Weight: 377 aa, 43 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC000887
• Gene Symbol: ERGIC2
• Gene ID (NCBI): 51290
• RRID: AB_2098440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96RQ1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ERGIC2, also named as Endoplasmic reticulum-Golgi intermediate compartment protein 2 or PTX1, is a 377 amino acid protein, which belongs to the ERGIC family. ERGIC2 is ubiquitously expressed in many tissues. ERGIC2 localizes in the endoplasmic reticulum-Golgi intermediate compartment membrane and may play a role in transport between endoplasmic reticulum and Golgi. ERGIC2 is involved in protein transportation, and participated in the elongation factor 1 alpha regulated mechanisms.