CoraLite®594-conjugated ERK1/2 Monoclonal antibody

ERK1/2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4G8A11

Cat no : CL594-66192

Synonyms

ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3



Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66192 targets ERK1/2 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERK1/2 fusion protein Ag20350
Full Name mitogen-activated protein kinase 3
Calculated molecular weight 43 kDa
GenBank accession numberBC013992
Gene symbol MAPK3
Gene ID (NCBI) 5595
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 ERK1/2 antibody CL594-66192Download protocol
FC protocol for CL594 ERK1/2 antibody CL594-66192Download protocol
Standard Protocols
Click here to view our Standard Protocols